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|“||Nothing is true, everything is permitted||„|
|~ Assassins motto|
The Assassins, or the Assassin Brotherhood, are the main protagonists in the Assassin's Creed video game series.
The bloodline of the original Assassins was formed millennia ago, after members of the First Civilization conceived children with humans. These children had the appearance of humans, but were gifted with extra senses from the precursor race, such as Eagle Vision. However, throughout the years, these senses were spread thin into the genetic pool, and became dormant in many humans. Only those with high concentrations of First Civilization genetics could freely use the power in the modern era, or those who gained the ability through the Bleeding Effect.
Regardless of ability, many of the resulting humans eventually banded together to accomplish the same goals shared with their modern-day descendants: the protection of the free will for all people, and the removal of individuals who would threaten the freedom of others.
Republic of Rome
During the Roman Republican era, the Assassin Order was a secret enemy of the SPQR – the Senatus Populusque Romanus – a precursor to the Templar organization that composed the ruling body of the Roman Republic. Little is known of the Assassin Order's hierarchy and activities during this time, barring a few major assassinations. In 44 BCE, forty Assassins, who had previously been part of the Roman senate, plotted the assassination of the Roman dictator, Gaius Julius Caesar.
Marcus Junius Brutus was tasked with plotting the assassination by Gaius Cassius Longinus, after seeing visions granted by the First Civilization in the Colosseum Vault. Twenty-three of the forty Assassins stabbed Caesar to death at the Roman senate. Following this event, the Assassins went their separate ways, but most were eventually tracked down and killed by Caesar's allies. Brutus himself committed suicide before the Battle of Philippi, which his forces eventually lost. Afterward, his Brothers unsuccessfully tried to revive him with the Shroud of Eden.
In 30 BCE, Cleopatra, Caesar's former lover and Queen of Egypt, was killed by the Egyptian Assassin Amunet with a venomous snake. Years later, on 24 January 41 CE, Roman Emperor Caligula was stabbed to death by the Assassin Leonius under unknown circumstances.
In 259 CE, the Assassins Accipiter and Aquilus safeguarded an Ankh for the Assassin Order, which was operating under the alias Liberalis Circulum. Aquilus' father, Lucius, initially held the Ankh, but when he was killed by Templars, Aquilus fought to retrieve it. He uncovered a Templar traitor, Faustinus, who pointed to Roman senator Caïus Fulvus Vultur as the ringleader before Aquilus eliminated him.
Though he was able to kill and retrieve the Ankh from Vultur, Aquilus died a captive of the Prefect of Lugdunum. Accipiter, Aquilus' cousin, managed to save Aquilus' wife, Valeria, and she told him of the work that they were doing. Together, the two recovered the Ankh, and Valeria interred it in a shrine to Aquilus' memory.
High Middle Ages
During the Middle Ages, the Templars became a public knightly order under the name "Knights Templar". In the year 1090, control of the Assassins fell to Hassan-i Sabbāh, who in turn changed the Assassin Order into a public organization. Under his command, public assassinations occurred much more often and the people were encouraged to stand up to their oppressors, realizing that they were not on their own.
During the 1170s, the Assassins were at odds with the Saracens; in 1174, thirteen Assassins were sent to the Sultan Saladin's camp close to Damascus to assassinate him. They successfully infiltrated his tent, but were detected once inside. One Assassin was killed by a Saracen general and the others were executed upon trying to escape.
In 1176, Saladin led a siege on the Assassins' fortress at Masyaf. After the location of Saladin's tent was revealed by the Assassin spy Ahmad Sofian, Umar Ibn-La'Ahad was sent to leave a feather and a dagger with a threatening message at Saladin's bedside. Despite a careful approach, he was ultimately detected and killed a Saracen noble to make his escape. Ahmad Sofian confirmed Umar's identity under torture, and Umar gave himself up to execution to spare Ahmad's life.
In 1189, the Assassins were betrayed by a man named Haras, who captured Mentor Al Mualim and three other Assassins, and allowed Crusaders into Masyaf's village. Under the leadership of young Assassin Altaïr, members of the Brotherhood were able to force the Crusaders back. Altaïr personally assassinated Haras and saved Al Mualim, earning the rank of Master Assassin.
The next year, the Assassins began to hunt for the Chalice, an object rumored to be so powerful that it could end the Crusades. Al Mualim wanted to keep it out of Templar hands, and sent Altaïr to recover it. Investigating the Chalice's location, he eventually tracked it to the Temple of Sand, but found the Templar leader Basilisk had removed it. As the structure began to crumble, Basilisk revealed that the Chalice was not an object, but a woman, then left Altaïr to die. However, Altaïr escaped and hunted Basilisk, uncovering the Templar plan to poison the water supply of Acre.
After foiling this plot, Altaïr followed the trail of the Chalice to Jerusalem and recognized her to be Adha, a former acquaintance of his. On Adha's instruction, Altaïr killed Harash, a traitor to the Assassin Order. Altaïr then planned to run away with Adha, but she was soon captured by Basilisk and transported to an unknown location. Years later, Altaïr found her dead, and began hunting the individuals responsible for her murder.
In 1191, the Assassins were still in a secret battle with the Templars and their leader, Robert de Sable. It was during the Third Crusade that the Assassins learned of the Templars' interest in an ancient artifact hidden within the catacombs of Solomon's Temple. A mission to retrieve the artifact was organized, and though it was successful, it led to the death of one Assassin, the disfigurement of another, and the arrival of the Templar army at the gates of Masyaf. The Assassins were nearly overwhelmed by the following battle, but were ultimately able to drive the Templars from the fortress and village.
Following the assault, the Assassin blamed for prompting the assault – Altaïr Ibn-La'Ahad.
This was a view shared by Al Mualim, who agreed with their goals, but felt that their attitude of "the end justifies the means" undermined the good they did. As his mission progressed, Altaïr sought to know what tied his victims together, and the discovery that they were all Templars reinforced his determination to bring an end to their plot.
By fall of 1191, Robert de Sable and his associates had been eliminated. However, it was soon discovered that Al Mualim was secretly a former member of de Sable's coalition and wished to use the Apple of Eden to force his rule upon the world. He was killed during a confrontation with Altaïr, who subsequently took on the position of Mentor of the Levantine Order. With full control over the Assassins, Altaïr set out to liberate the island of Cyprus from the Templars, who had recently purchased it from King Richard I. After assassinating the Templar Grand Master Armand Bouchart and discovering the Templar Archive Altaïr returned to Masyaf with Maria Thorpe, de Sable's former disciple and Templar deserter.
During this time, Altaïr began to write a detailed Codex based on information gleaned from the Apple of Eden. These secrets profoundly affected the Order, as new assassination techniques and equipment changed the way the organization operated. In 1209, Altaïr began to send his Assassins to cities throughout the world, after he was made aware of their existence by the Apple of Eden.
In the year 1217, Altaïr set out with his wife Maria and son Darim to kill Genghis Khan, leaving Malik Al-Sayf as the Order's temporary leader. Khan's army had advanced westward rapidly, and Altaïr suspected that he possessed a Piece of Eden. On their journey, the family met with Mongolian Assassin Qulan Gal, and the group worked together to end the threat. Ultimately, Darim and Qulan succeeded in killing the Khan in 1227.
On their return to Masyaf, Altaïr found that his rival Abbas Sofian had taken over the Order, imprisoning Malik and killing Altaïr and Maria's youngest son, Sef. Shortly after Altaïr's arrival, Malik was killed as well. Abbas then attempted to take the Apple from Altaïr, leading to Maria's death at the hands of Swami, Abbas' lackey.
Altaïr and Darim fled Masyaf and Altaïr fell into a deep depression, obsessing over the Apple and ultimately driving Darim and Sef's family away. Continued study of the Apple led him to Memory Seals, and taught him how to make several advanced gadgets. Recovering his mental fortitude, Altaïr returned to Masyaf in 1247 and found it in disrepair. He rallied Assassins loyal to him and Malik, and turned several of Abbas' henchmen to his side. Together they took the fortress, and Altaïr executed Abbas with the Hidden Gun, reclaiming the title of Mentor.
During this time, the Mongol threat continued advancing west, and Altaïr ordered his Assassins to abandon Masyaf and carry on their activities in secret. He trained and initiated the travelers Niccolò and Maffeo Polo into the Order in 1257, then tasked them with creating Assassin guilds throughout the Old World. He then gave them his Codex and the Memory Seals for safekeeping in Constantinople before locking himself in the empty Masyaf library.
Late Middle Ages
- "In this modern age, we are not as literal as our ancestors; but our seal is no less permanent..."
During the Late Middle Ages, the Order continued to thrive, despite being forced to adopt an even more secretive and isolated existence. When the Renaissance – a cultural movement – started in Italy, the Assassin Order primarily focused themselves in Italy, where the headquarters of the Templar Order were also located.
In the early 14th century, the Assassins manipulated King Philip IV of France to try and destroy the Templars. They made the Church brand the Templars heretics, turning their own weapon against them, with the intention of finishing off the Templars. They managed to have the Templar Grand Master, Jacques de Molay burned at the stake, however, his nine most trusted men had escaped after he had passed on the knowledge of the ancients, and their Order continued to survive.
By 1321, various members of the Assassin Order had successfully integrated themselves into the everyday life of middle Italy, widely unknown to the average folk. That same year, a young sailor by the name of Domenico, the son of an Assassin, was apprenticed to the poet and Assassin Dante Alighieri, who had been tasked with transporting Altaïr's Codex from Venice to Spain.
Upon Dante's death, however, the undertaking fell instead to Domenico. During the voyage, pirates under the employ of the Templars ambushed the ship looking for the Codex, consequently killing Domenico's wife. Fortunately for the Assassins, Domenico managed to break apart the Codex and hide its pages before he and his son were forced overboard.
Eventually returning to Venice, Domenico found his father and his patron, Marco Polo, an ally of the Assassins, dead. Thus, Domenico emptied the bank accounts of Messer Polo, and traveled to the city of Florence under the adopted name of Auditore. He eventually constructed the Villa Auditore in the city of Monteriggioni, which became the center of the Assassin Order in Italy up to the year 1500.
Despite the order "modernizing" since the 12th century, they still retained many of the rituals and practices, albeit altered. The practice of removing one's ring finger had been ceased, although the seal of the Order was branded upon the finger instead with a heated iron. Although significantly fewer in number, the Assassins were supported by the courtesans, thieves and mercenaries of Florence and Venice; the guilds of which were each run by a member of the Order.
During this time period, the Assassins entered another span of increased hostility with the Templars, who were attempting to overthrow the ruling House of Medici in Florence, and the Doge of Venice. Having already killed the Duke of Milan in 1476, Rodrigo Borgia, the Grand Master of the Templar Order, brought the noble families of Pazzi and Barbarigo together to accomplish his goals.
Entering into conflict with the Assassins, Borgia dealt a crippling blow to the Order in 1476, when he had the experienced Assassin Giovanni Auditore da Firenze and his two sons executed, Federico and Petruccio, although a third son, Ezio, had managed to escape. With the aid of Ezio, the Assassins were able to counter Borgia's plot over a twenty-three year campaign, defeating him at every turn.
In 1487, the Order of Assassins learned that the Templars had retrieved another Apple of Eden from the island of Cyprus, and deemed its capture necessary. Following the artifact from L'Arsenale di Venezia, Ezio managed to overpower and replace the Templar guard carrying the artifact. Under this guise, Ezio met with Borgia himself, and the two engaged in battle.
Borgia fled upon the arrival of other Assassins, and in doing so, the artifact fell into the hands of their Order. The Assassins of this age, however, were unaware of the nature of the artifact, although they did recognize it as one of the Pieces of Eden mentioned within Altaïr's Codex.
By 1500, right after Cesare Borgia besieged the city of Monteriggioni, murdering Mario Auditore and once again claiming the Apple for the Templars, Ezio Auditore had moved to Rome, meeting up with Niccolò Machiavelli and laying the foundations of a new "brotherhood" of Assassins. Utilizing the mercenaries, thieves and courtesans of Bartolomeo d'Alviano, La Volpe and Claudia Auditore da Firenze respectively, Ezio's brotherhood worked to combat the influence and power of the Borgia family, and by extension, the Papacy itself.
Headquartered in an underground storeroom owned by Fabio Orsini, on Tiber Island in the center of Rome, the Assassins began to spread their influence throughout the city, recruiting Rome's own citizens as Assassin apprentices under Ezio, eventually becoming inducted and branded into the Order as Assassins themselves. Under the instruction of Ezio, many Assassin recruits undertook a variety of missions across Europe and Asia in cities such as London, Paris, Barcelona, Lisbon, Moscow and Calicut.
There, the recruits killed and sabotaged Templars, the Templars' allies, and corrupt men in power, as well as defending and aiding their Assassin brothers and sisters, along with any willing to stand against the Borgia and the Church.
Over a period of three years, Ezio's brotherhood whittled away the Borgia family's power, and with the murder of Rodrigo Borgia at the hands of his own son, and the arrest of Cesare by the Papal Guard of Pope Julius II, the Templars' influence over the city of Rome was shattered.
Three years after Siege of Viana in 1507, when Ezio ended the threat of Cesare Borgia for once and all, the Assassin Mentor traveled to the ancient fortress of Masyaf, on a pilgrimage to learn about the origins of the Order. When he arrived, he discovered that the fortress had been overrun by the Templars, who were searching for the five keys, which would unlock a secret library beneath the fortress of Masyaf.
With one seal already in the Templars' possession, Ezio learned that the remaining four were hidden throughout the city of Constantinople. From there, Ezio made his way to the city in the hopes of obtaining the remaining seals.
When he arrived in Constantinople, Ezio was welcomed by the guild there, led by Yusuf Tazim, and learned how to use their unique weapons. With the knowledge made known to him that the Templars had taken over the Byzantine remnants, and in the recent years, had been attempting to overthrow the Ottomans, Ezio began to take a leading role in the conflict.
He retook all the Assassin Dens around the city and trained seven recruits to the rank of Master Assassin, assigning each to one of the dens. The Constantinople guild of Assassins also worked to help liberate and spread their Order's influence in other cities, namely Bursa, Rhodes, Marseille, Damascus, Jerusalem, Madrid, Lisbon, Tunis, Tripoli, Athens, Algiers and Alexandria, where they slowly whittled away Templar control, rebuilt the cities and protected them from attacks by the Templars.
They also rescued captured Assassins in these cities, and helped those who opposed the Templars. During these ventures, the Assassins were responsible for destroying the compound of the Knights Hospitalier.
Together with Yusuf, Ezio protected the Ottoman prince Suleiman I from the Templars, while continuing his search for the keys. Befriending a Venetian lady, Sofia Sartor, she deciphered a map containing the location of the keys. After finding four of the keys to Altaïr's library, Ezio traveled to Cappadocia – after asking Yusuf to protect Sofia at her shop – to hunt down Manuel Palaiologos and find the final key. On doing so, he discovered that Manuel was not the true leader of the Byzantines, but that Suleiman's uncle – Ahmet – was.
On his return to Constantinople, Ezio made his way to Sofia's shop, only to find that Yusuf had been killed and Ahmet had captured Sofia, demanding the keys in exchange. However, after a full scale assault on the city's Arsenal, Ezio chased him over the countryside, culminating with Ahmet's brother, Selim I, killing Ahmet by throwing the man from a cliff.
From there, Ezio and Sofia traveled to Masyaf and unlocked the library of Altaïr, where Ezio attempted to communicate with Desmond. Deciding that his mission was over, Ezio left behind his sword and Hidden Blade, resigning his life as an Assassin.
Once he had returned to Italy, Ezio appointed the Assassin Lodovico Ariosto as the Mentor of the Italian Assassins and then retired from the Order, marrying Sofia Sartor and having two children with her, Flavia and Marcello Auditore, while settling down on a Tuscan vineyard.
As the Templars had been severely weakened in Europe, the Assassins began collecting and studying several Pieces of Eden. In 1510, Francesco Vecellio hunted down Niccolò di Pitigliano, recovering the Shroud of Eden.
By 1519, Giovanni Borgia was secured a position aboard Hernán Cortés' ship, which was sailing to the New World. After the Spanish troops began to ransack Tenochtitlan, Giovanni recovered a Crystal Skull from the Mayan city, assassinating one of their priests to obtain it. On bringing it to Paracelsus for study, the Assassins found that it was not one of the Pieces.
In 1527, Giovanni accidentally read a tome belonging to Paracelsus and succeeded in crafting the Philosopher's Stone. After discovering that the tome was one half of the Book of Abraham, he searched for the other half with Maria Amiel, Paracelsus' assistant, but only found a translation. However, he did develop a relationship with Maria, with the two of them becoming romantically involved and later marrying each other.
In 1542, after studying the Crystal Skull over a long period of time, Giovanni found out that it could be used to communicate over large distances, seeing the face of a Chinese man in his mind.
Around this time, the Chinese Assassins were fighting desperately against the Templars. Several Chinese Emperors came to power through Templar support, who would then orchestrate the mass killings of Assassins. One such emperor was Yongle, who was killed by the Assassin Li Tong in 1424.
When Emperor Jiajing came to power, he nearly destroyed the Brotherhood in China, though a handful of Assassins fled west for support. Shao Junand her Mentor escaped to Italy, though Jiajing's men killed Jun's Mentor along the way, leaving her to fend for herself. With no alternative, Jun went to Ezio to seek guidance and support.
Though he initially wanted nothing to do with the Assassins anymore, he eventually relented and taught her how to rebuild her Brotherhood. Shao Jun, armed with this knowledge and a box that Ezio told her to open only if she lost her way, traveled back to China with the vow to rebuild the Chinese Brotherhood, no matter how long it took.
As the reach of the Old World spread to the New, the Assassins followed to ensure they were able to protect it from any advances the Templars made. In 1503, the Italian Assassins under Ezio were sent to recover a map of the New World made by Piri Reis. Using this map, they too began to send their forces to America. The first Assassin to ever reach the New World was John de la Tour, who set up a base for the Brotherhood there.
In 1510, the Assassins in Florida recruited Alonso Carlo and his adopted son, Miguel Ramón Carlo de Lugo, to their cause. In 1521, Carlo was tasked with stopping the explorer Juan Ponce de León from reaching the Fountain of Youth, where they knew he would find a Piece of Eden. While Miguel distracted León in conversation, a poisoned arrow killed the explorer, following which Miguel escaped.
In the mid-1730s, Achilles Davenport was recruited to the Colonial branch of the Assassin Order and became its Mentor in 1746. The Templars, headed by Haytham Kenway, nearly decimated this branch of Assassins in 1763, wiping out most of its members and nearly all of its records. The exact nature of the attack still remains unknown, but it left at least one survivor, Achilles, who disbanded the Order and went into retirement.
Years later, Achilles mentored the young Assassin Ratonhnhaké:ton, who ultimately destroyed the Colonial Rite of the Templar Order through his actions during the American Revolutionary War. Ratonhnhaké:ton also rebuilt the province's Brotherhood, by recruiting several citizens of New York and Boston.
- "In the old days, that usually meant killing anyone who became too powerful or greedy. [...] These days, it means subversion of established regimes. [...] Countries. Corporations. We change the system from within."
- ―"The Mentor", in 2000, on the Assassin Order.
During the mid-19th century, at the start of the Sikh Empire, the Indian Assassin Arbaaz Mir and ancestor to Monima Das was active in the search for a powerful Piece of Eden known as the Koh-i-Noor. During this period the Indian Assassins were led by the Assassin Hamid in the capital city of the Sikh Empire, Amritsar.
By the late 19th century, the Russian sect of the Assassin Order, the Narodnaya Volya (People's Will), played a vital role in overthrowing the Tsarist rule over Russia. The Assassins strongly assisted their close ally, Vladimir Lenin, the leader of the Bolsheviks – as Lenin's brother was an Assassin himself.
Due to the efforts of the Assassin Nikolai Orelov, the Templar-affiliated Tsar Alexander III died of kidney failure and a Staff of Eden was destroyed in the Tunguska explosion. Without power, Tsarevich Nicholas II was removed from the throne and Russia became communistic.
By the 20th century, the tactics and practices of the Assassin Order had changed greatly. Whilst the Order of Assassins had kept to their policy of remote camps and isolation, the Templars had begun to infiltrate society further; taking the form of many publicly known corporations such as NASA, Kraft and BP, which all secretly linked to the shadow-corporation Abstergo Industries.
The Templars Winston Churchill, Joseph Stalin, Franklin D. Roosevelt, and Adolf Hitler planned a war, World War II, which would place the Templars in control. At the end of the war, Hitler was supposed to kill his double, created by the Piece of Eden in his control, and then escape. However, the Assassins spoiled this victory by assassinating Hitler as he exited the bunker that his body double was placed in.
The Assassins relied less upon killing powerful or greedy individuals, and more on changing the "established regime" from within, using politics as well as the blade to achieve their aims, such as rigging elections to keep Templars out of office.
The American presidential election of 2000 was one such election, with the Templars attempting to place their puppet George W. Bush into power, and the Assassins backing Al Gore.
By the year 2000, the Hidden Blade had become a largely ceremonial weapon. In the meantime however, the Templars continued to search for and investigate the various Pieces of Eden they discovered throughout this period, whilst the Assassins continued their war against them; notably being responsible for the destruction of at least one Templar-controlled base in Siberia in 1908, known in public as the Tunguska explosion.
However, by the year 1985, the Templars, under the guise of their front-company Abstergo Industries, had begun kidnapping Assassins or those related to them in an attempt to locate even more Pieces of Eden.
That same year, a ten to eleven year old child, a descendant of the Assassin Nikolai Orelov, was kidnapped by Abstergo's Lineage Research and Acquisition department and entered into the Animus Project, later being codenamed "Daniel Cross". Years later, in 1998, Daniel joined the Assassin Order as a prospective, but with a mission to find the then-current Mentor.
Two years later, he was granted his wish and inducted into the Order by the Mentor himself; Cross subsequently murdered the Mentor with the very Hidden Blade he had been bequeathed by his victim just moments before, as a result of a neurological impulse instilled in him by Warren Vidic during his time as an Abstergo captive.
Cross' actions directly led to the fall of the Assassin Order, as he compromised virtually all of the Assassin camps around the world that he had visited in search for the Mentor upon his programmed return to Abstergo Industries, which forced the Assassins even further into hiding. Other notable Assassins held captive by Abstergo included Clay Kaczmarek and Desmond Miles – both descendants of Ezio Auditore and Adam.
After the Mentor's death, William Miles began to take a prominent role in the Order's functioning, and became their de facto leader. It was his decision of raising Lucy Stillman outside the Order after the age of seventeen, so that all her ties to the Assassins were cut and she could infiltrate Abstergo.
The Assassins attempted to spy on Abstergo using moles several times, but the people they sent always turned out to be strong and got themselves killed, or too weak and easily manipulated by the Templars.
Sometime around 2008, William also recruited Clay Kaczmarek to the Order. Tasked with infiltrating Abstergo and discovering information about them, Clay returned successfully with a memo detailing the allocation of Abstergo Industries' budget, along with their funding of the Animus Project. With this information known to the Assassins, a plan was devised for him to get caught by their Lineage Research and Acquisition department as Subject 16, and was to be rescued later by Lucy.
However, years of isolation had caused her to turn against the Assassins, and once Clay learned of her defection through hacking into Abstergo's systems and uncovering security footage, she did not help him escape when the time came, leading to his subsequent suicide.
- "We lost two more teams last night. That's eight more of us just... gone."
- ―Lucy Stillman, 2012.
Later on, the Templars tracked down Desmond Miles, William's son, who had left the Order in 2003. Using Desmond, Abstergo managed to obtain a map of the locations of the Pieces of Eden across the globe, and subsequently begun their attempts to claim them. When Desmond began to suffer from the Bleeding Effect, Warren Vidic told Lucy to take him somewhere where he would feel safe, as part of a plan code-named "Project Siren".
Not long after, the Assassins were able to free Desmond with the "aid" of Lucy, as well as obtain their own copy of the map. Following this, Desmond agreed to aid the Assassins in their quest to defeat Abstergo, mostly for revenge, but also for closure.
Utilizing the Animus 2.0, a copy of the machine used by Abstergo to explore his genetic memories previously, Desmond followed the memories of Ezio Auditore, adopting his skills in combat and freerunning, while the rest of the Order concentrated to locating the Pieces of Eden before their Templar counterparts.
Despite this, however, the war with the Templars had taken a turn for the worse. With their numbers dwindling, the Order of Assassins was in danger of losing the war. At least two known teams were reported to have been killed, one in Brisbane, Australia, and another in Whistler, Canada.
As technology progressed, so did the weapons used against the Assassins. By 2012, the Templars were using cell phone towers and satellites to track them down. As a result, Desmond and his group had to take refuge within the Sanctuary, deep beneath the city of Monteriggioni. By this point, the Order was using at least one e-mail network, known as the Hephaestus Email Network, in order to facilitate communications.
In the latter half of 2012, an Assassin team made up of Lucy Stillman, Rebecca Crane, Shaun Hastings and Desmond Miles discovered the Colosseum Vault – after Desmond had relived Ezio's memories in Rome through the Animus – and infiltrated it, securing the Apple of Eden that Ezio had hidden there centuries before. On contact with First Civilization artifact, Desmond's body was taken over by the ancient being Juno, who forced him to stab and kill the Assassin-traitor, Lucy.
This indirectly helped to ensure that the Templars could not get their hands on Ezio's Apple of Eden and kept Desmond safe from Templar influence from within the Assassins. Due to this event, the team and the Apple were brought to William Miles and the Order, in turn securing one of the ancient artifacts for the Order's cause.
Also, around this time, an Assassin team in Moscow were chasing a potential lead on a Piece of Eden. However, as William Miles stated, this was some of the only good news they had got. There were teams in Osaka and São Paulo. In Osaka, the team was being tailed, so they had gone dark. In São Paulo, a leak was confirmed and the team was working on flushing it out.
The team in Denver were even worse off. They had lost contact without informing, and their safe house was empty. This meant that they were dead or captured, or that they had gone dark without notifying the Assassin headquarters.
After killing Lucy, Desmond entered a coma, and was put back into the Animus. He relived a restored memory sequence, which provided the Assassins with co-ordinates, leading to the Grand Temple. While most Assassins continued their fight with Abstergo, William's team – with help from Gavin Banks – reached New York, hoping to find the Temple and stop the end of the world.
Upon finding out that they needed a key, and three "batteries" to open the door in the Grand Temple, Desmond began to work on finding the key, by reliving memories that Juno told him to. While William's team began working on opening the Temple, he had other teams run interference, and tried to thwart Abstergo's attempts to find them, or other Pieces of Eden.
During this endeavor, several Assassins were killed by Abstergo agents, including Adriano Maestranzi. William was captured by Juhani Otso Berg when he went alone to Cairo to retrieve the third and final power source. This attempt to find Desmond and take the Apple from him ended in failure, as he assassinated both Daniel Cross and Warren Vidic, killing the men responsible for nearly destroying the order 12 years previously, and foiling the Eye-Abstergo through these actions.
Returning to the Temple, Desmond fully reactivated it and discovered the Eye, a device that would save the world from the Second Disaster. Juno urged Desmond to activate the Eye, but Minerva asked him not to, saying that the Eye's activation would also kill Desmond and release Juno from her prison.
While Minerva and her brethren tried to save the world, Juno was planning to use their technology to conquer it, so she was imprisoned. Minerva showed Desmond that if he let the Second Disaster happen, the world would be devastated, but humanity would endure. Desmond would become a legendary figure that would rebuild the world. However, upon his death, his ideals would be misinterpreted by future generations, bringing about the fall of civilization, and "what once was, shall be again".
Believing the world would have a chance at defeating Juno, Desmond told William, Shaun and Rebecca to leave and prepare for the incoming struggle. Desmond then activated the Eye and the world was indeed saved, but as he was informed, Desmond died and Juno was released from her prison.
- Darius/Artabanus : A Persian Proto-Assassin who assassinated proto-Templars ally Xerxes I of Persia.
- Amorges: A former member of the Persian elite and a close friend of Darius. After the assassination of King Artaxerxes I, he betrayed Darius and his group and joined the Order of the Ancients.
- Pactyas: A former member of the Persian elite and and a group of freedom fighters alongside Amorges and Artabanus. After the assassination of King Artaxerxes I, he betrayed Darius and his group and joined the Order of the Ancients.
- Kassandra: A Spartan mercenary and the granddaughter of King Leonidas I of Sparta who fought during the Peloponnesian War. She is the main protagonist of Assassin's Creed: Odyssey.
- Iltani: A Babylonian Proto-Assassin who assassinated proto-Templars ally Alexander the Great.
- Wei Yu: A Chinese Proto-Assassin who assassinated proto-Templars ally Qin Shi Huang.
- Bayek: A Medjay who found Hidden Ones (Assassin Brotherhood). He is the main protagonist of Assassin's Creed: Origins.
- Amunet/Aya: A Greco-Egyptian agent to Cleopatra VII, the last pharaoh of Egypt, and the wife of Bayek who assassinated Order of the Ancients puppet Cleopatra. One of the founders of Hidden Ones (Assassin Brotherhood).
- Pasherenptah: A high priest in Memphis during the reign of Pharaoh Ptolemy XIII and one of the founding members of the Hidden Ones, the organization that would later transform into the Assassin Brotherhood.
- Phanos the Younger: An actor and playwright active in Alexandria during the late Ptolemaic dynasty, and one of the founding members of the Hidden Ones, the organization that would later transform into the Assassin Brotherhood.
- Phoxidas: A Greek Nauarchos in Cleopatra's Navy and one of the founding members of the Hidden Ones, the organization that would later transform into the Assassin Brotherhood.
- Tahira: One of the founding members of the Hidden Ones, the organization that would later transform into the Assassin Brotherhood.
- Lugos: One of the Roman founders of the Liberalis Circulum, an earlier iteration of the Assassin Brotherhood in Rome.
- Publius Volumnius: A Roman philosopher during the 1st century BCE.
- Marcus Junius Brutus: A politician of the late Roman Republic who assassinated Order of the Ancients ally Julius Caesar.
- Gaius Cassius Longinus: A Roman Senator and the brother in-law of Marcus Junius Brutus who began to plot Caesar's assassination.
- Kashta: A member of the Hidden Ones who operated alongside Tahira in the Sinai Peninsula.
- Maqwat: A member of the Hidden Ones who operated in the Sinai Peninsula alongside Tahira.
- Osorkon: A member of the Hidden Ones who was part of Tahira's bureau on the Sinai Peninsula.
- Shaqilat: A Nabatean warrior who resided on the Sinai Peninsula along with her husband. After her husband was crucified by the Roman legate Tacito, Shaqilat dedicated herself to liberating the slaves at the Klysma Quarry from the Romans.
- Paanchi: A member of the Hidden Ones who operated in Sinai in 38 BCE.
- Kawab: The son of Taharqa who was the steward of Letopolis and a member of the Order of the Ancients.
- Caesarion: The last Pharaoh of Egypt and the eldest son of Queen Cleopatra. The queen asked the Hidden One to protect and train him.
- Leonius: A Roman Assassin who assassinated Templar puppet Caligula.
- Lucius: A Roman Assassin and the father of Aquilus.
- General Accipiter: An Aleman general who was in a leading position of the Alemanni forces, leading them in battle across Gaul, hoping to conquer the region.
- Aquilus: A Gallo-Roman Assassin in Lugdunum who was tasked with the assassination of several high-ranked individuals within the Roman Empire. He is one of the main protagonists of Assassin's Creed (French comics).
- Cuervo: An Iberian member of Liberalis Circulum.
- Vitus: A Roman magister who lived in the Province of Britain during the 5th century.
- Li E: A member of the Chinese Brotherhood of Assassins who lived in the Tang dynasty during the 8th century.
- He Shi: A member of the Chinese Assassins during the Tang dynasty and wife of Yan Jiming.
- Basim: A member of the Hidden Ones who operated during the 9th century and befriend of Sigurd Styrbjornson, the son of King Styrbjorn of Forberg, and his younger brother, Eivor.
- Hytham: A member of the Hidden Ones who were brought to Norway by Sigurd Styrbjornson.
- Rayhan: The Mentor of the Levantine branch of the Hidden Ones in the late 9th century.
- Thorvald Hjaltason: An Icelandic skald and proto-Assassin active during the 10th century.
- Torgny the Lawspeaker: The old advisor of King Eric of Sweden and the Mentor of the Viking proto-Assassin Brotherhood active during the 10th century.
- Hassan-i Sabbāh: The leader of Levantine Assassins during the late 11th and early 12th century.
- Hassan the Younger: The leader of the Levantine Brotherhood of Assassins in Alamut from 1162 until 1166.
- Rashid ad-Din Sinan (Al Mualim): The Mentor of Levantine Assassin until his death in 1191, and the tutor to his eventual successor: Altaïr Ibn-La'Ahad. Rashid's desire to obtain an Apple of Eden led to him secretly working with the Knights Templar, sworn enemies of his own Order.
- Altaïr Ibn-La'Ahad: A Syrian Assassin during the Middle Ages and, from 1191 until his death, the Mentor of the Assassins in the Levant. He is the main protagonist of Assassin's Creed.
- Maria Thorpe: An English noblewoman living during the Middle Ages, who joined the Templar Order prior to the Third Crusade. She betrayed the Templar Order and joined the Assassin Order in 1193. She married with Mentor Altaïr Ibn-La'Ahad.
- Malik Al-Sayf: The head of the Assassin bureau in Jerusalem in 1191, later the right-hand man of Altaïr Ibn-La'Ahad and one of the Mentor of the Levantine Assassins.
- Umar Ibn-La'Ahad: A Levantine Master Assassin and the father of Mentor of the Levantine Assassins, Altaïr Ibn-La'Ahad.
- Ahmad Sofian: A member of the Assassin Order in the Levant, and the father of Abbas Sofian.
- Faheem Al-Sayf: A Levantine Master Assassin and the father of Malik and Kadar Al-Sayf.
- Maud: A Christian member of the Levantine Assassin Brotherhood at the time of the Crusades and the mother of Altaïr Ibn-La'Ahad.
- Kadar Al-Sayf: A Levantine Assassin and the younger brother of Malik Al-Sayf, and the uncle of Malik's son Tazim Al-Sayf.
- Abbas Sofian: A Syrian Assassin during the Middle Ages, and the Mentor of the Levantine Assassins from 1227 until his death.
- Swami: A Levantine Assassin during the High Middle Ages. After Abbas Sofian became the head of the Order's council, Swami became his right-hand man.
- Damascus Bureau leader: The Assassins Bureau leader of Damascus and a Rafiq.
- Jabal: A Rafiq of the Assassin Order and the Bureau leader in Acre during the Third Crusade.
- Kadar: An Assassin Rafiq in Jerusalem during the Third Crusade, who had been tasked with monitoring the Crusaders' regime in the city.
- Rafik: A silk merchant of Damascus, who served as an Assassin Rafiq of the city during the time of the Third Crusade.
- Labib: The fighting instructor of both Altaïr Ibn-La'Ahad and Abbas Sofian during their formative years as Assassins.
- Rauf: A Levantine Assassin who served as a combat instructor for new recruits.
- Darim Ibn-La'Ahad: A Levantine Assassin and the eldest son of Mentor Altaïr Ibn-La'Ahad, and his wife Maria Thorpe.
- Sef Ibn-La'Ahad: A Levantine Assassin, and the youngest of Altaïr Ibn-La'Ahad and Maria Thorpe's sons.
- Tazim Al-Sayf: A Levantine Assassin and the son of Malik Al-Sayf.
- Vahid Ahmedi: A Levantine Assassin during the High Middle Ages.
- Fahd El Fehmi: A Levantine Assassin during the Middle Ages.
- Raşit El Zübeydi: A Levantine Assassin during the Middle Ages.
- Hamid (Tyre): A carpet merchant in Tyre that served as the Assassin Rafiq of the city during the Third Crusade.
- Hazad: An Assassin and a shopkeeper in Jerusalem at the time of the Third Crusade.
- Haras: An Assassin apprentice who, after becoming dissatisfied with his slow progression through the Assassin ranks, defected to the Templar Order.
- Harash: The keeper of the Levantine branch of the Assassin Order, and thus Rashid ad-Din Sinan's second-in-command until his death. He secretly defected to the Templar Order, providing its members with valuable inside information.
- Jamal: A Levantine Assassin who had gotten into contact with the Templar Order.
- Farim: A Levantine Assassin and father of Swami, Abbas Sofian's right-hand man.
- Qulan Gal: A Mongolian Assassin who assassinated Jochi Khan, Genghis Khan and Chagatai Khan.
- Nergüi: A Mongolian Assassin and the apprentice of Qulan Gal who assassinated Ögedei Khan, Alexander Nevsky and Hülegü Khan.
- William of Cassingham: An English Master Assassin and expert archer who fought for King John during the First Barons' War, as opposed to the barons supported by Robert Fitzwalter and the English Brotherhood.
- Robert Fitzwalter: A English baron and crusader, responsible for starting a campaign to unite the English Barons against the despotic King John.
- Egyptian Assassin (1250): An Egyptian Assassin was tasked to bring the Scepter of Aset to the Mamluks as a gift to help them in their slave revolt against the rule of the Ayyubid tyrants.
- Kang: The Mentor of the Chinese Brotherhood of Assassins, active during the 13th century.
- Zhang Zhi's father: A commander in the army of the Song Dynasty who was trained by the Mentor Kang and in turn trained his daughter Zhang Zhi to also become an Assassin.
- Zhang Zhi: An initiate of the Chinese Brotherhood of Assassins during the Song Dynasty.
- Thomas de Carneillon: A banker and a Mentor of the French Assassins in the late Middle Ages. He attacked on the Templar Order which was leading by Jacques de Molay.
- Guillaume de Nogaret: A French jurist who became the councilor and keeper of the seal to King Philip IV of France, and the Mentor of the French Brotherhood of Assassins.
- Yolande of Aragon: A throne claimant and titular queen regnant of Aragon, titular queen consort of Naples, Duchess of Anjou, Countess of Provence, and regent of Provence during the minority of her son. She was also the Mentor of the French Brotherhood of Assassins, using her influence over her dull son-in-law, the future King Charles VII of France, to prevent the Templars controlling England to take back France after the purge of the Templars a century before.
- Jean de Metz: A French nobleman who is known primarily for his role in the exploits of Joan of Arc.
- René d'Anjou: The count of Piedmont, Duke of Bar, Duke of Lorraine, Duke of Anjou, Count of Provence, King of Naples, titular King of Jerusalem and Aragon including Sicily, Majorca and Corsica.
- John II of Alençon: A Duke of Alençon during the Hundred Years' War.He was a French Assassin novice who was trained alongside Jeanne d'Arc and Gabriel Laxart but eventually joined the Templar Order after the Brotherhood left Jeanne to be executed.
- Numa Al'Khamsin: An Egyptian Assassin who started a quest to regain the Scepter of Aset from the Templars. He is one of the main protagonists of Assassin's Creed (French comics).
- Ali Al-Ghraib: The young disciple of Egyptian Assassin Numa Al'Khamsin.
- Assassin Elder (1340): A member of the Assassin Brotherhood in Egypt.
- Niccolò Polo: A Venetian merchant, explorer and one of Founder of Italian Assassins and Ottoman Assassins.
- Maffeo Polo: A Venetian merchant, explorer and one of Founder of Italian Assassins and Ottoman Assassins.
- Marco Polo: An explorer and a merchant for the Venetian Republic of Italy.
- Dante Alighieri: An Italian poet of the Middle Ages, known as the writer of the Divine Comedy.
- Domenico Auditore: The founder of the Auditore family and the architect of the Villa Auditore in the town of Monteriggioni.
- Renato Auditore: The only known son of the Assassin Domenico Auditore and his wife Isabetta.
- Fang Xiaoru: An orthodox Confucian scholar of the Ming dynasty and the leader of the Chinese Assassins.
- Li Tong: A Chinese Assassin who assassinated Templar ally Yongle Emperor.
- Lukas Zurburg: A German member of the Northern European Brotherhood of Assassins. Lukas was recruited into the Assassin Order at the age of sixteen after a group of Assassins saved him from a thief, who tried to rob Lukas while traveling.
- Benedicto: The Mentor of the Spanish Brotherhood of Assassins around the time of the Granada War.
- Luis de Santángel: A baptized Jew born in Valencia and the finance minister of King Ferdinand II.
- Raphael Sánchez: The treasurer of Queen Isabella I during the late 15th century.
- Aguilar de Nerha: A Spanish Assassin during the 15th century. He is the main protagonist of Assassin's Creed (Movie).
- Maria: A Spanish Assassin during the 15th century who wore robes bearing Moorish influence and worked with another Assassin, Aguilar de Nerha.
- Mario Auditore: The leader of the Italian Assassins from at least 1476 to his death, a condottiero, the ruler of Monteriggioni from 1454 until his death, who was also responsible for the training of his nephew, Ezio Auditore da Firenze.
- Giovanni Auditore da Firenze: A Florentine nobleman, the head of the Auditore family in Florence and the father of Mentor Ezio Auditore da Firenze.
- Maria Auditore da Firenze: A Florentine noblewoman during the Renaissance and alongside her husband Giovanni.
- Federico Auditore da Firenze: A Florentine noble and the elder brother of Mentor Ezio Auditore da Firenze, trained into the Brotherhood by his father.
- Ezio Auditore da Firenze: A Florentine nobleman during the Renaissance, unbeknownst to most historians and philosophers, and one of the Mentor of the Italian Assassins. He joined the Assassins Order in 1488. He is the main protagonist of Assassin's Creed II, Assassin's Creed Brotherhood and Assassin's Creed Revelations.
- Niccolò Machiavelli: An Italian philosopher, humanist, writer, a diplomat, political philosopher, musician, playwright and one of the Mentor of the Italian Assassins.
- Lodovico Ariosto: An Italian poet who succeeded Ezio Auditore da Firenze as the Mentor of the Italian Assassins in 1513.
- Claudia Auditore da Firenze: A Florentine noblewoman during the Renaissance and the sister of Mentor Ezio Auditore da Firenze.
- Paola: The leader of the Florentine courtesans and owner of La Rosa Colta.
- La Volpe: The leader of the Florentine and Roman thieves.
- Antonio de Magianis: The leader of the Thieves Guild located in the Italian city of Venice during the 15th century.
- Sister Teodora Contanto: The leader of the Venetian courtesans who founded brothel La Rosa della Virtù.
- Bartolomeo d'Alviano: An Italian condottiero and the leader of Venetian and Roman mercenaries.
- Rosa: A member of the Venetian Thieves Guild led by Antonio de Magianis, and later the Madame of the Rosa in Fiore.
- Lo Sparviero: A Italian Assassin active in Rome, Florence and Monteriggioni during the Renaissance.
- Vincenzo: A member of the Italian sect of the Assassin Order during the 15th century.
- Rinaldo Vitturi: A member of the Italian sect of the Assassin Order during the 15th century, and was commonly known as the Keeper of the Shroud.
- Pietro Bembo: An Italian scholar, poet and cardinal.
- Baldassare Castiglione: An Italian diplomat, author and soldier.
- Ridolfo Fioravant: An Italian architect who was sent as a spy to Moscow, where he was hired to work on the Kremlin alongside fellow Assassin Pietro Antonio Solari.
- Pietro Antonio Solari: A Swiss-Italian architect stationed in Moscow, Russia.
- Perotto Calderon: An Italian assassin and the assumed father of Giovanni Borgia who became traitor of the Assassins Order.
- Francesco Vecellio: An Italian painter who was raised and trained by Perotto Calderon, an Assassin secretly posing as a courier for the Borgia family.
- Cipriano Enu: An African member of the Italian Assassins.
- Tessa Varzi: An Assassin during the Renaissance. She was a member of the Italian Assassin team that included Francesco Vecellio and Cipriano Enu, with whom she completed various missions across the country.
- Alif: A Moroccan pirate who join Italian Assassins during the Renaissance.
- Alessandra d'Avanzago: A noblewoman in Venice during the Renaissance.
- Claudio: The son of a spice merchant who gained confidence and slowly made his way up to the Venetian aristocracy.
- Dariâ: A member of Persian Assassins and Italian Assassins. She was a descendant of the Venetian explorer Marco Polo.
- Clara: A member of the Italian Brotherhood of Assassins who operated in Rome during the Renaissance.
- Beatrice: A member of the Italian Brotherhood of Assassins who operated in Rome during the Renaissance.
- Domenico: A Venetian monk during the Renaissance.\
- Bastiano da Mezzo: A Venetian actor and nobleman during the Renaissance.
- Giovanni Borgia: An Italian nobleman who born in Templars family, House of Borgia.
- Hiram Stoddard: A ruthless British Assassin active during the 16th century who was also a close ancestor to Thomas Stoddard.
- Desiderius Erasmus: A Dutch Renaissance humanist, Catholic priest, theologian and the leader of the Northern European Assassins.
- Helene Dufranc: A French rogue born into a family of Assassins.
- Ishak Pasha: An Ottoman general, statesperson, Grand Vizier and the leader of the Ottoman Assassins.
- Yusuf Tazim: The leader of Ottoman Assassins, operating primarily in the Empire's capital of Constantinople.
- Piri Reis: A famed admiral, cartographer and a Master Assassin of the Ottoman Assassin Brotherhood.
- Castor: A Master Assassin in Rhodes who was killed by the Knights Hospitalier.
- Kasim: A Ottoman Master Assassin and a lieutenant under their leader Yusuf Tazim.
- Dogan: A Master Assassin and the leader of the Ottoman branch of the Assassin Order, following the death of his predecessor, Yusuf Tazim.
- Heyreddin: An Ottoman Assassin during the early 16th century, fighting as a lieutenant under Yusuf Tazim and later Dogan.
- Ece Ashkar: An Ottoman Assassin in Constantinople who took part in a number of missions on the orders of Yusuf Tazim, prior to Ezio Auditore's arrival to the city in 1511.
- Azize: An Ottoman Assassin who was saved by Mentor Ezio Auditore da Firenze.
- Evraniki: An Ottoman Assassin during the early 16th century, fighting under its leaders Yusuf Tazim and later Dogan.
- Yerman Ragar: A Ottoman Assassin, living in Constantinople.
- Irini: An Ottoman Assassin during the early 16th century, fighting under its leaders Yusuf Tazim and later Dogan.
- Vali cel Tradat: A Wallachian noble, former Assassin and member of the Templar Order.
- Mujir: The leader of the Levantine Brotherhood of Assassins in the early 16th century who was arrested by the Mamluks in order to intimidate the Assassins in Jerusalem, as the Mamluks were fearful of their recruiting efforts.
- Zhu Jiuyuan: A Master Assassin and Mentor of Chinese Assassins. He notably trained Shao Junfrom initiate to the full rank of Assassin during the secret war in Beijing, after Wang Yangming's disappearance to escape the Great Rites Controversy.
- Wang Yangming: An idealist Neo-Confucian philosopher, official, educationist, calligraphist and general of the Ming dynasty of China. He was also the Mentor of Chinese Assassins.
- Shao Jun: A Chinese Assassin and and the main character of Assassin's Creed Chronicles: China. A former concubine of the Zhengde Emperor, she was rescued by the Assassins after the emperor's death, and devoted her life to the Order as a result. He became Mentor of Chinese Assassins in 1532.
- Miguel Ramón Carlo de Lugo: An Assassin member in Florida.
- Iskender: The Mentor of the Egyptian Assassins during the early 16th century.
- Hattori Hanzō: A famous samurai and ninja who was a vassal for Tokugawa Ieyasu, the last of the three unifiers of Feudal Japan.
- Mogami Yoshiaki: A powerful daimyo of northern Japan.
- Yamauchi Kazutoyo: A Japanese general.
- Yamauchi Taka: A Japanese monk active during the Sengoku period of Japan. He was a friend of fellow Assassin Hattori Hanzō, and after Hanzō's death, worked closely with Tokugawa Ieyasu.
- Liu Yan: A Chinese Assassin. In June 1582, she was tasked to bring a Sword of Eden to a safe location after it was retrieved from Oda Nobunaga by Yamauchi Taka.
- Hattori Masanari: The son of Hattori Hanzō and a vassal for Tokugawa Ieyasu.
- Don Gonzalez Pardo: A Spanish nobleman active in Peru during the Spanish conquest of the Inca Empire in the 16th century.
- Rose Galloway: An Assassin initiate and the daughter of a wealthy family in London, England who betray the Brotherhood.
- Omar Khaled: A blind English Assassin who lived during the late 17th century.
- William Lowndes: A British Whig politician who sat in the English and British House of Commons from 1695 to 1724. He was Secretary to the Treasury of Great Britain under King William III and Queen Anne.
- Maréchal: A Master Assassin of the French Brotherhood.
- Louis-Joseph d'Albert de Luynes: A French aristocrat, member of the French Army and politician who was sent by their Mentor to recruit a skilled epeist and singer, Julie d'Aubigny.
- Mireille: The leader of the French Assassins and the lover of the famous bandit Cartouche.
- Louis-Dominique Cartouche: A legendary French bandit, gang leader and womanizer, known to steal from the rich and give to the poor.
- Julie d'Aubigny: A renowned swordswoman and singer who became a member of the French Assassins.
- Thomas Stoddard: A Master Assassin of the Assassin Brotherhood, well-known for being a tracker of Pieces of Eden.
- Jennifer Querry: A British nurse active during the Salem witch trials.
- Phillip Randall: The Mentor of the British Brotherhood of Assassins in 1714.
- Duncan Walpole: A British Master Assassin during the early 18th century. Duncan later chose to betray his Order and join the Templars based in the West Indies.
- Bahlam: The Mentor of the Caribbean Assassins and the father of Ah Tabai.
- Ah Tabai: A Mayan Assassin and the Mentor of the Caribbean Assassins.
- Edward Kenway: A Welsh-born British privateer-turned-pirate and a Assassin. He then joined the Assassin Order in 1721 and hunted down the Templars in the region and entrusted the Observatory to the Assassins.He became an Assassin leader and Master Assassin of British Assassin. He is the main character of Assassin's Creed IV: Black Flag.
- Adéwalé: A former slave-turned-pirate from Trinidad, and later a member of the Assassin Order. He is the main protagonist of Freedom Cry. He briefly set aside his duties as an Assassin in order to aid the Maroon rebellion in Saint Domingue.
- Mary Read: An English pirate, trained by the Mentor of the Caribbean Assassins, Ah Tabai. She was also one of the founders of the Pirate Republic of Nassau.
- Antó: A Master Assassin that led the Assassin bureau in Kingston.
- Opía Apito: A Master Assassin of Taíno origin affiliated with the Caribbean Brotherhood.
- Rhona Dinsmore: A Scottish Master Assassin who led the bureau of Assassins in Havana during the early-to-mid 18th century.
- Vance Travers: The head of the Assassin bureau in Nassau during the early 18th century. He was quickly fell in love with Templar Jing Lang. He betrayed the Assassin Order and ordered Edward Kenway to kill his brother Upton Travers.
- Upton Travers: The head of the Assassin bureau in Nassau alongside his brother Vance Travers.
- Samuel Bellamy: An English pirate who operated in the West Indies during the early 18th century.
- Dakodonou: An Individual sold into slavery at fourteen, keeping his beliefs and desires to create a better life for his kin a secret.
- Maria van Antwerpen: A Dutch soldier who enlisted and served in the army as a man under the name Jan van Ant.
- Miko: The alias of the leader of the British Assassins alongside Edward Kenway, and the holder of the Grand Temple Key.
- Louis Mills: A crew member aboard the Providence.
- François Mackandal: A Maroon leader and the Mentor of Haitian Assassins during the 18th century.
- Vendredi: A Maroon member of the Haitian Brotherhood of Assassins under the leadership of Mentor François Mackandal. In 1751, he was tasked with exploring a First Civilization Temple in Haiti.
- Baptiste: The Saint-Dominguan voodoo leader of a cult of former slaves in the Louisiana Bayou. He was Assassin turncoat and the ally of Templar Order.
- Agaté: The Mentor of the Assassin Brotherhood in Louisiana.
- Aveline de Grandpré: An Assassin of French and African heritage, who lived in the area of New Orleans during the middle of the 18th century. She is the main character of Assassin's Creed III: Liberation. In 1759, after having lost sight of her mother, she mistook another woman to be her parent and reached out to her, attracting the attention of a group of slavers. She was rescued by an Assassin called Agaté, who recruited Aveline into the Assassins Brotherhood and became her Mentor.
- Gérald Blanc: An accountant in the employ of Philippe de Grandpré.
- Babatunde Josèphe: A Haitian Assassin and the son of Adéwalé and Bastienne Josèphe.
- Nicolas de Saint-Prix: A French Assassin who acted as a representative of the French Assassin Council, sending a letter to Achilles Davenport,Mentor of the Colonial Brotherhood.
- Salah Bey: The captain of Nāder Shāh's guards who assassinated Nāder Shāh with his fellow Assassins.
- John de la Tour: A French-Canadian Assassin who played a pivotal role in the foundation of Colonial Assassins.
- Achilles Davenport: The Mentor of Colonial Assassins from 1746 until 1763.
- Liam O'Brien: A Colonial Assassin operating in the British Colonies during the Seven Years' War and a childhood friend of Shay Cormac.
- Louis-Joseph Gaultier, Chevalier de la Vérendrye: A French-Canadian of noble upbringing operating in North America during the Seven Years' War. He captained the Gerfaut, a Man O' War.
- Kesegowaase: A Wolastoqiyik member of the Colonial branch of the Assassin Order during the Seven Years' War.
- Hope Jensen: A Colonial Assassin operating in North America during the Seven Years' War.
- Shay Cormac : An Assassin-turned Templar who became an Assassins hunter. He was the main protagonist of Assassin's Creed: Rogue.
- Michael Crawley: A skilled Colonial Assassin who defected from the Brotherhood and started a new life in Old Growth Forest.
- Benjamin Tallmadge, Sr.: A priest from Connecticut who narrowly survived the Templar purge of the Colonial Brotherhood in 1763.
- Ratonhnhaké:ton (Connor): A Master Assassin and Assassin leader of Colonial Assassins. He is the main protagonist of Assassin's Creed III.
- Robert Faulkner: A veteran sailor and first mate aboard the Aquila, during Ratonhnhaké:ton's captaincy.
- Stephane Chapheau: A French-Canadian chef in Boston during the American Revolutionary War. He was aided by the Assassin Connor when the British forces attempted to collect taxes from him, and was later recruited by Connor as an apprentice.
- Deborah Carter: A member of Colonial Assassins who was recruited by Assassin Connor.
- Doctor Jamie Colley: An American doctor who was recruited by Assassin Connor.
- Duncan Little: A former Irish priest who was recruited by Assassin Connor.
- Clipper Wilkinson: A noted sharpshooter, who had specialized in using a musket. He was recruited by Assassin Connor in Boston's southern district during the American Revolutionary War.
- Jacob Zenger: A former Hessian in the German Army, who was recruited into the Assassin Order by Assassin Connor in New York.
- Atasá:ta: An individual of Kanien'kehá:ka descent who was raised away from his nation by his Assassin father and taught in the art of murder, using the skills to survive.
- Caleb Garret: A gunslinger who joined the American Revolutionary War and the Assassin Order, after witnessing his friends being burned alive by a group of Templars.
- Joe: A believed to be a psychologically unstable mercenary who murdered civilians in the streets of New York, but was in fact an extraordinary member of the Assassin Brotherhood, with justice as his primary motivator, killing those who abused women.
- Duncan McGill: A Scottish highlander who was exiled from his home country, and arrived in the British colonies of America.
- William de Saint-Prix: An Assassin born to a wealthy family of French descent.
- Patience Gibbs: The teenage leader of a band of escaped slaves, as well as the carrier of a mysterious charm. She was recruited into the Assassin Brotherhood by Aveline de Grandpré in 1784.
- Honoré Gabriel Riqueti, comte de Mirabeau: A French statesman and author, as well as a leader of the French Revolution during its early stages. He was also the Mentor of the French Brotherhood of Assassins, who sought to establish peace with the Parisian Rite of the Templar Order and its Grand Master, François de la Serre in the early days of the revolution.
- Pierre Bellec: A French-Canadian Master Assassin and a former colonial soldier.
- Guillaume Beylier: the son of a wealthy plantation owner and a slave who born in the French colony of Saint-Domingue. He became a Master Assassin after several years of service.
- Hervé Quemar: A Master Assassin, part of the French Brotherhood's Assassin Council and a lawyer.
- Sophie Trenet: A French Master Assassin and member of the Assassin Council that lead the Parisian Brotherhood during the French Revolution. She was also a playwright, essayist and pamphleteer who fought with passion for the freedom of the oppressed classes.
- Arno Dorian: A French-Austrian Master Assassin during the French Revolution and the main character of Assassin's Creed: Unity. He sought redemption by joining the Assassins Order in 1789 and working to expose the true instigators of the Revolution, whilst also avenging him in the process.
- Charles Dorian: A French Assassin and the father of Arno Dorian who entrusted with protecting an ancient artifact known as the Precursor box in 1776.
- Charlotte Gouze: A French Assassin and the manager of the Café Théâtre owned by Arno Dorian.
- Augustin Grisier: A French Assassin of African heritage and the fencing master in the Café Théâtre owned by Arno Dorian.
- Didier Paton: A French spy working for Maximilien de Robespierre during the Reign of Terror.
- François Auverlot: A French Assassin active during the French Revolution.
- Louise Auverlot: A French Assassin operating during the French Revolution.
- Camille Berteau: A highly skilled assassin agent operating during the French Revolution.
- Pierre Berteau: A French Assassin operating during the French Revolution.
- Hubert Besy: A French Assassin operating during the French Revolution.
- Simon Bonhomme: A French Assassin operating during the French Revolution.
- Guillaume Bourgeois: A French Assassin operating during the French Revolution.
- Jacques Cagnard: A French Assassin operating during the French Revolution.
- Angélique Chevrier: A French Assassin operating during the French Revolution.
- Charles Chevrier: A French Assassin operating during the French Revolution.
- Geneviève Dalle: A French Assassin operating during the French Revolution.
- Joseph Deshais: A French Assassin operating during the French Revolution.
- Antoine Faroy: A French Assassin operating during the French Revolution.
- Antoine Fieve: A French Assassin operating during the French Revolution.
- Nicolas Fribourg: A French Assassin operating during the French Revolution.
- Paul Ganchard: A French Assassin operating during the French Revolution.
- Alexandre Garnier: A French Assassin operating during the French Revolution.
- François Garnier: A French Assassin operating during the French Revolution.
- Jacques Girard: A French Assassin operating during the French Revolution.
- Gabriel Godin: A French Assassin operating during the French Revolution.
- Anne Grelet: A French Assassin operating during the French Revolution.
- Pierre Guilbert: A French Assassin operating during the French Revolution.
- Louise Harly: A French Assassin operating during the French Revolution.
- Philippe Hélissant: A French Assassin operating during the French Revolution.
- Claude Hure: A French Assassin operating during the French Revolution.
- Catherine Imbert: A French Assassin operating during the French Revolution.
- Charles Imbert: A French Assassin operating during the French Revolution.
- Edmé Leblanc: A French Assassin operating during the French Revolution.
- Simon Leblanc: A French Assassin operating during the French Revolution.
- Laurent Lemaire: A French Assassin operating during the French Revolution.
- Claude Lejeune: A French Assassin operating during the French Revolution.
- Madeleine Leroux: A French Assassin operating during the French Revolution.
- Marguerite Mignot: A French Assassin operating during the French Revolution.
- Paul Morot: A French Assassin operating during the French Revolution.
- Élisabeth Roger: A French Assassin operating during the French Revolution.
- Laurent Rousseau: A French Assassin operating during the French Revolution.
- Germain Savin: A French Assassin operating during the French Revolution.
- Thomas Simon: A French Assassin operating during the French Revolution.
- Denis Varlet: A French Assassin operating during the French Revolution.
- Cartouche II: The second incarnation of Cartouche, and the successor of Louis-Dominique Cartouche.
- Cartouche III: The third incarnation of Cartouche, and the heir of Louis-Dominique Cartouche.
- Doctor Claude Chemin: A French doctor.
- Couillaud: A novice of the French Brotherhood of Assassins active during the French Revolution who betrayed the Order.
- Pierre Gard: A French Assassin who was murdered in the French Revolution.
- Camille Hautcoeur: The daughter of a New France peasant and a French soldier, who learned to use a sword in her teens and later vowed to hunt down and kill those who murdered her parents.
- Marguerite Huet: A French Assassin operating during the French Revolution.
- Le Téméraire: A French Assassin operating during the French Revolution.
- Brasseur: An apprentice of Guillaume Beylier that had been tasked to find the Roi des Thunes in the Cour des Miracles.
- Al Mualim (1794): A Mentor of Assassins based in Cairo, Egypt during late 18th century.
- Bernard Ruddock: A former member of the British Brotherhood of Assassins.
- Eseosa: The leader of the Haitian Assassins, a leading figure of the Haitian Revolution and the grandson of Adéwalé and Bastienne Josèphe.
- Toussaint Louverture: The leader of the Haitian Revolution against the French colonists.
- Dutty Boukman: A Jamaican-born Haitian slave and one of the leading figures of the Haitian Revolution.
- Elsie: An Assassin active in Haiti during late 1791.
- Fabien: An Assassin active in Haiti during late 1791. He prevented Elsie from saving their fellow Assassin from execution.
- Jeannot Bullet: A leader of the slave rebellion of Haitian Revolution who betrayed the Order.
- Georges Biassou: A former slave on Saint-Domingue and a leader of the Haitian Revolution. He was Assassin turncoat.
- Jean-François Papillon: A leader of the slave rebellion of the Haitian Revolution who betrayed the Order.
- Ludger Duvernay: A Canadian journalist who posted a number of articles accusing the Canadian administration of serving the Château Clique, the leaders of the Templars at that time.
- Ahkbar: An agent of the Sultan of the Ottoman Empire, Selim III, during the 19th century. He betrayed the Assassin Brotherhood.
- Michel Reuge: A British Assassin who found the Aegis, the armor of the Precursor Minerva, near Bath.
- Hamid (Amritsar): The Mentor of the Indian Assassins.
- Arbaaz Mir: A Kashmiri member of the Indian Brotherhood of Assassins, the main character of Assassin's Creed Chronicles: India, and one of the most prominent Master Assassins of the 19th century.
- Pyara Kaur: The daughter of Maharaja Kharak Singh of the Sikh Empire, the granddaughter of Maharaja Ranjit Singh. She was married to the Master Assassin Arbaaz Mir, with whom she had a son, Jayadeep Mir (Henry Green).
- Emmett Frye: A member of the British Brotherhood of Assassins at some point in history.
- Ethan Frye: A schoolmaster during the Victorian era, as well as the husband of Cecily Frye and the father of twins Jacob and Evie.
- Cecily Frye: A British Assassin during the Victorian era, the wife of Ethan Frye and the mother of Jacob and Evie Frye.
- American Assassin (1852): An American Assassin was active in the Great Basin Desert during the California Gold Rush.
- Ajay: An Indian Assassin and the custodian of the Assassin headquarters under Amritsar. He was Assassin turncoat.
- Kulpreet: An Indian Assassin active in India during 19th century.
- American Mentor: An Afro-American Assassin and the Mentor of the American Brotherhood during the American Civil War.
- Varius: An American Assassin active in New York City during the American Civil War.
- Eliza: An Afro-American servant, who, along with her father, Abraham, was employed by William M. Tweed during the American Civil War.
- Jayadeep Mir (Sir Henry Green): An Indian immigrant in London during the 1860s, who became the leader of the Assassins in the city and mentor to the twins Jacob and Evie Frye.
- Sir Jacob Frye: A British Master Assassin, Assassin leader of British Assassins and leader of the Rooks active in London during the Victorian era, and the younger twin brother of Evie Frye. He is one of main character of Assassin's Creed Syndicate. He later became a member of Queen Victoria's Order of the Sacred Garter.
- Dame Evie Frye: A British Master Assassin, the leader of the Rooks active in London during the Victorian era, and the elder twin sister of Jacob Frye. She is one of main character of Assassin's Creed Syndicate. She later became a member of Queen Victoria's Order of the Sacred Garter.
- George Westhouse: A British Assassin who eventually began undertaking missions with Ethan and Cecily's twins, Jacob and Evie.
- Moina Mathers: The wife of Samuel Liddell Mathers and the "Seeress" of the Hermetic Order of the Golden Dawn who became an Assassin turncoat.
- Annie Horniman: An English theatre patron manager and occultist.
- Jack the Ripper: An unidentified serial killer who became infamous following a series of gruesome murders of young women. These women were fellow Assassins disguised as prostitutes who attempted to stop his spiral of madness.
- Mary Ann Nichols (Assassin): An unidentified British Assassin initiate was active during the Whitechapel murders and the first real victim of the Assassin turncoat and serial killer Jack the Ripper.
- Lydia Frye: A British Assassin, the wife of Sam Crowder and the granddaughter of Jacob Frye.
- Sam Crowder: A British soldier and the husband of Lydia Frye who enlisted in the army during the war.
- Assassin medic (1914): A British Assassins, and served in the British Army during the First World War. He assassinated German general and Templar Erich Albert.
- Sergei: A Russian Assassin and a scientist at an Assassin laboratory in Protvino.
- Galina Voronina's grandmother: A Russian Assassin and the wife of fellow Assassin Sergei.
- Andrei Orelov: A member of the Narodnaya Volya, a group of Russian Assassins, and the father of Nikolai Orelov.
- Nikolai Orelov: A Russian Master Assassin during the Russian Revolution and the main character of Assassin's Creed Chronicles: Russia.
- Aleksandr Ulyanov: A Russian revolutionary and older brother of Vladimir Lenin. He was also a personal friend of Nikolai Orelov.
- Ilia: A Russian Assassin and a friend of Nikolai Orelov.
- Irish Assassin: An Irish Assassin operated in Ireland during the country's War of Independence from 1919 to 1921.
- American Assassin (1920s): An American Assassin operated in the United Statesduring the Jazz Age in the 1920s.
- Ignacio Cardona: A Spanish Assassin active during the Spanish Civil War.
- Dwight Adams: An American Assassin part of Ignacio Cardona's cell during the Spanish Civil War.
- Miguel Carasso: A Spanish priest active during the Spanish Civil War.
- Norbert Clarke: A member of British Assassins who was active during the 20th century.
- Glaucia Acosta: a member of the Spanish Brotherhood of Assassins active during the Spanish Civil War. She was the ex-lover of fellow Assassin Ignacio Cardona. However, after witnessing the power of the Koh-i-Noor, she betrayed the Brotherhood and defected to the Instruments of the First Will.
- Eddie Gorm: A member of the British Brotherhood of Assassins who operated in Europe during World War II. He is the main protagonists of Assassin's Creed: Conspirations.
- Robert Burton: A British spy and doctor who operated in Europe during World War II.
- Colonel Boris Theodore Pash: A colonel in the United States Army during World War II. He officially led the Alsos Mission, aimed at spying on secret German operations regarding their atomic bomb project as the Allies were developing their own program, the Manhattan Project.
- Julia Dusk: An American spy who operated in Europe during World War II.The lieutenant Dusk was part of an US operation commanded by fellow Assassin, colonel Boris Pash, who was tasked to gather intelligence on the atomic project of the Nazis as the Americans were developing their own.
- Alekseï Gavrani: A former Soviet hitman recruited by the CIA during the Vietnam War.
- Julia Gorm: A member of the Assassin cell called Bloodstone Unit and the daughter of Eddie Gorm.
- William Greer: An agent of the United States Secret Service, best known for having driven President John F. Kennedy's automobile in the motorcade through Dealey Plaza in Dallas on November 22, 1963, where the president was assassinated. The Unit was a cell of Assassin extremists, who were in fact the ones who killed Kennedy in order to steal his Apple of Eden for Pash's experimentations.
- Zenia: A member of Bloodstone Unit and the mother of the director of the Clinique des Rameaux Nathalie Chapman.
- William King Harvey: The director of the Central Intelligence Agency who founded the Bloodstone Unit in the Assassin Brotherhood led by Boris Pash. After secretly defecting to the Templar Order, he performed as a double agent within the brotherhood.
- Mentor (2000): The Mentor of the Assassin Order in Worldwide who was killed by Daniel Cross.
- Daniel Cross: A Templar and sleeper agent tasked with the infiltration of the Assassin Brotherhood, in order to assassinate its Mentor.
- Paul Bellamy: The director of an Assassin training camp located near the city of Philadelphia.
- Hannah Mueller: An Assassin during the late 20th century and early 21st century, and was based in an Assassin compound near Philadelphia.
- Janice: A member of the modern-day Assassin Order, who led an Assassin cell in Whistler, Canada.
- Adriano Maestranzi: An Assassin during modern times, who led a cell in Florence, Italy.
- Harlan Cunningham: An Assassin in who operated out of Florence, Italy as part of a cell led by Adriano Maestranzi.
- Arend Schut Cunningham: A former mixed martial arts champion and Assassin from the Netherlands, as well as the husband of Harlan Cunningham.
- Jasdip Dhami: An Indian Assassin and the brother of Siobhan Dhami. In 2013, the siblings were tasked with locating the Koh-i-Noor diamond. He defected to the Instruments of the First Will, a cult venerating Juno, and is now fighting against his former brothers.
- Siobhan Dhami: An Indian Assassin and an employee at MysoreTech. She was tasked with locating the Koh-i-Noor diamond.
- Dinesh: A member of the Assassin Brotherhood, operating as part of a cell in India.
- Kenichi Mochizuki: A Japanese Assassin, the husband of Saeko Mochizuki, and one of the first Mentors of Gavin Banks.
- Saeko Mochizuki: The leader of the Onmoraki-Gumi and the Mentor of the Assassin Brotherhood in Osaka, Japan. The Onmoraki-Gumi were a faction of the yakuza located in Osaka, Japan.
- Kiyoshi Takakura: A member of the Onmoraki-Gumi, the Assassin cell located in Osaka, Japan.
- Gavin Banks: The former leader of the Assassin Order, stationed on a ship named the Altaïr II.
- Emmanuel Barraza: An ex-soldier, serves as the ship's armorer and weapons instructor and Assassin stationed aboard the Altaïr II.
- Eric Cooper: A transgender Scotsman, a member of the Initiates group and the Assassin Order, as well as the navigator and supervisor aboard the Altaïr II.
- Doctor Stephanie Chiu: A member of the Initiates group and part of the Assassin Order stationed aboard the Altaïr II. She serves as the ship's medical officer.
- Susan Drayton: A Canadian member of the Assassin Order and the captain of the Altaïr II.
- Emmett Leary: A former employee of Abstergo Industries and a member of the Assassin Order stationed on the Altaïr II. He serves as the cell's hacker and enjoys smoking recreational marijuana.
- Akaki Ninidze: An Assassin and the chief engineer aboard the Altaïr II.
- Nodar Ninidze: An Assassin and the chief steward aboard the Altaïr II.
- Clay Kaczmarek: An assassin who had been kidnapped by Abstergo Industries and forced to relive his ancestors' memories in the Animus.
- William Miles: An Assassin who acted as a contact for Assassin teams spread throughout the world. Based in the Assassin headquarters. In 2015, he became had become the new Mentor of the Assassins Order in Worldwide.
- Desmond Miles' mother: The wife of Assassins Mentor, William Miles, The mother of Desmond Miles and a member of the Assassin Order.
- Desmond Miles: An Assassin and was descended from many legendary assassins, including Aquilus, Altaïr Ibn-La'Ahad, Ezio Auditore da Firenze, Edward Kenway, and Ratonhnhaké:ton. He is the central protagonist of the Assassin's Creed video game series.
- Lucy Stillman: A member of the Assassin Order and a genetic memory researcher for Abstergo Industries' Animus Project. She was double agent between Templars and Assassins.
- Shaun Hastings: An Assassin, a historian, a scholar and a hacker who gave tactical guidance to numerous Assassin teams across the globe.
- Rebecca Crane: An Assassin, a hacker, the creator of the Animus 2.0 and a member of Lucy Stillman's team, which concentrated on acquiring an Apple of Eden before Abstergo Industries.
- Bishop: An Assassin and a hacker who is responsible for directing Assassin activity across the globe, often to sabotage the Templars.
- Helix initiate: An individual who was occasionally contacted by the Assassin known as Bishop.
- Galina Voronina's mother: The Mentor of the Russian Assassins, also working as a scientist in a cell located in an old Assassin laboratory in Protvino, outside of Moscow.
- Avdotya Voronina: An Assassin and part of a cell operating from an Assassin Science City in Protvino, Moscow. Born to an Assassin scientist, she was also the twin sister of Galina, and granddaughter of Sergei and another scientist.
- Christian: An Assassin active in the 21st century and the lover of another Assassin, the veteran Joseph.
- Charlotte de la Cruz: An employee of Malta Banking Corporation from San Diego who undertook Assassin training in 2015.
- Kody: The technician of the Assassin team led by Xavier Chen and consisting of Galina Voronina, and later Charlotte de la Cruz.
- Xavier Chen: An Assassin and the head of a cell operating in California.
- Joseph: An Assassin active in the 21st century who decided to take revenge on the Assassins by teaming up with the Templars.
- Moussa: An assassin and a descendant of Assassin turncoat Baptiste.
- Griffin: A member of the Assassin Brotherhood active during the 21st century.
- Yanmei: A member of the Assassin cell operating in China.
- Barindra Mitra: An Assassin and the leader of a cell in Asia.
- My'shell Lemair: A member of Erudito and one of the new field agents trained after the reorganization of the hacker collective in 2016.
- Layla Hassan: A former employee of Abstergo Industries who forced to leave Abstergo following a Templar attempt on her life, she was offered a position with the Assassins by their leader, William Miles.
- Alannah Ryan: An Assassins working as part of Layla Hassan's cell in searching for the Staff of Hermes Trismegistus in 2018.
- Victoria Bibeau: A psychiatrist at Abstergo Entertainment and a former Templar who was secretly affiliated with the Assassin Order.
- Yuri: The mother of Tomo Sakagawa and oversaw the Assassins' training camp on the Island of Kyushu.
- Hajime Shimada: A Japanese Assassin who was a part of the Assassins' training camp on the island of Kyushu.
- Tomo Sakagawa: A Japanese Assassin who posed as "Dr. Florent Carpentier" in the Clinique des Rameaux in Switzerland.
Allies and puppets
- Fajera: A Romani woman that assumed the profession of a circus dancer in Damascus.
- Adha: A woman from the Middle East who possessed mysterious powers.
- Masun: A resident and preacher of the village of Masyaf. He and an Assassin, Jamal, defected to the Templar cause.
- Mukhlis: A tradesman who became the steward of Assassin Altaïr Ibn-La'Ahad, following the retaking of Masyaf.
- Alexander of Limassol: A member of the Cypriot Resistance, who was most notable for assisting the Assassin Altaïr Ibn-La'Ahad as the latter chased the Templars to Cyprus.
- Markos: A member of the Cypriot Resistance, whose aims lay against the Templar Order.
- Osman: A Templar soldier based in Cyprus, and secretly a member of the Cypriot resistance.
- Barnabas: The Cypriot Resistance leader in Kyrenia, who actively supported in the revolt against the Templars, though he was eventually assassinated because of his efforts.
- Philip IV of France: The King of France from the Capetian dynasty that reigned from 1285 until his death in 1314.
- Pope Clement V: A French prelate and later Pope from 1305 until his death.
- Uberto Alberti: The Gonfaloniere of Florence during the Renaissance who betrayed his close friend and Assassin Giovanni Auditore da Firenze.
- Leonardo da Vinci: An anatomist, sculptor, cartographer, painter, botanist, engineer, architect, and mathematician of the Renaissance. He was a close friend and ally to the Florentine Assassin Ezio Auditore da Firenze.
- Orazio: A condottiero and one of Mario Auditore's most loyal soldiers.
- Lorenzo de' Medici: An Italian statesman and de facto ruler of the Florentine Republic during the Italian Renaissance.
- Ugo: A member of the Venetian Thieves Guild led by Antonio de Magianis during the 15th century.
- Agostino Barbarigo: The Doge of Venice from 1486 until his death in 1501 who later became ally of Templar Order.
- Piero de' Medici: The son of Lorenzo de' Medici and Signore of Florence from 1492 until 1494.
- Christopher Columbus: An Italian-born navigator, colonizer and explorer who worked with the nation of Spain.
- Isabella I of Castile: A Queen of Castile and León, and the wife of King Ferdinand II. She later became Templar puppet.
- Ferdinand II of Aragon: The King of Aragon, Sicily, Naples, Valencia, Sardinia, and Navarre.
- Muhammad XII of Granada: The Emir of Granada between 1487 and 1492.
- Caterina Sforza: The Countess of Forlì and Imola, and the daughter of Galeazzo Maria Sforza, the Duke of Milan.
- Margherita dei Campi: A countess of the city Rome during the Renaissance.
- Solari: The Madame of the Rosa in Fiore brothel in Rome, and the sister to Santino.
- Egidio Troche: A Roman Senator during the Renaissance in Italy.
- Pantasilea Baglioni: The wife of the Assassin Bartolomeo d'Alviano.
- Nicolaus Copernicus: A Renaissance astronomer, mathematician, and priest.
- Fabio Orsini: An Italian condottiero and a member of the Papal Guard. He was also the cousin of Bartolomeo d'Alviano.
- Pope Julius II: The Pope from 1503 to 1513, and was the successor of Pius III.
- Fiora Cavazza: A courtesan working in the Rosa in Fiore, who was recruited into the Templar Order by Cesare Borgia. She betrayed the Templar Order, joined forces with the Assassins and helped them take down the agents she had once collaborated with.
- Henry VII of England: The King of England and Lord of Ireland from 22 August 1485 until his death, and the first monarch of the House of Tudor.
- Conradus Celtis: A German Renaissance humanist scholar and Neo-Latin poet.
- Samis El Felipe: A King of Algiers, until Spain conquered the city in 1510.
- Blaise Legros: An opportunistic "captain for hire" who lent his abilities to anyone who would pay for them.
- Suleiman I: The tenth Sultan of the Ottoman Empire from 1520 until his death.
- Sofia Sartor: A Venetian-Ottoman bookshop owner, and a literary enthusiast.
- Selim of Algiers: An amir of Algiers, who proposed that he would turn a blind eye to the activity of the Assassins in the region, provided that a group of Ottoman Assassins would protect a community of Moors residing on the island of Penon from the Spaniards that occupied it.
- Pope Leo X: The Pope from 1513 to his death in 1521.
- Maximilian I, Holy Roman Emperor: The Emperor of the Holy Roman Empire from 1493 until his death.
- Hong Liwei: A low-rank eunuch of the Forbidden City who taught calligraphy to the Zhengde Emperor's concubines, and an informant and ally for the Chinese Brotherhood of Assassins living during the early 16th century.
- Don Pedro Navarro: A Spanish military engineer and general.
- Hayreddin Barbarossa: An Ottoman admiral and pirate famous for his activities along the Mediterranean Sea.
- Maria Amiel: A Hermetic practitioner, who lived in western Europe during the 16th century.
- Bombastus: A Swiss Renaissance physician, botanist, alchemist, astrologer, and general occultist.
- Tokugawa Ieyasu: The founder and first shogun of the Tokugawa shogunate of Japan, which ruled from 1600 until 1868.
- Honda Tadakatsu: A Japanese samurai, serving as one of Tokugawa Ieyasu's four generals, the "Four Guardians", along with Ii Naomasa, Sakakibara Yasumasa and Sakai Tadatsugu.
- Ii Naomasa: A general under the daimyo and shogun Tokugawa Ieyasu, regarded as one of the "Four Guardians" of the Tokugawa, along with Honda Tadakatsu,Sakakibara Yasumasa and Sakai Tadatsugu.
- Chaya Shirōjirō Kiyonobu: A Japanese silk merchant and information dealer, part of the famous Chaya Shirōjirō family of Kyoto and active during the Sengoku period.
- Elizabeth I of England: The Queen of England and Ireland, reigning from 17 November 1558 until her death.
- Birtwistle: The coxswain aboard Edward Kenway's ship, the Jackdaw.
- Hanley: The quartermaster aboard Edward Kenway's ship, the Jackdaw, following the resignation of Anne Bonny.
- Olivier Levasseur: A French privateer-turned-pirate of the Caribbean.
- Alonzo Batilla: A French pirate, active in the Caribbean, during the early 18th century.
- Anne Bonny: An Irish pirate who operated in the West Indies.
- Rose: A housemaid working for Emmett Scott, a Bristolian merchant, during the early 1700s.
- Bastienne Josèphe: The madam of La Dame en Rose, a brothel in Port-au-Prince, during the 1730s, and a supporter of Augustin Dieufort's Maroon rebellion.
- Augustin Dieufort: The leader of a Maroon rebellion in the area of Port-au-Prince, and quartermaster of the Experto Crede during the 1730s.
- Lucio Albertine: A scholar and a Corsican associate of the Assassins.
- Monica Albertine: A Corsican ally of the Assassin Order.
- Abigail Davenport: The wife of Master Assassin Achilles Davenport and the mother of their son, Connor Davenport.
- Louis XV of France: The King of France from 1715 to his death.
- Le Chasseur: A French spy operating in New York and the North Atlantic during the Seven Years' War.
- Philippe Beaubien: A Canadian fisherman in St. John's during the mid-18th century.
- Kaniehtí:io: A Kanien'kehá:ka warrior from the village of Kanatahséton.
- Gaspar Velasquez: A Spanish shipbuilder associated with the Assassin Order.
- Élise Lafleur: A smuggler who, together with her partner Roussillon, ran smuggling operations in the Louisiana Bayou during the 18th century.
- Roussillon: A smuggler that, together with his partner Élise Lafleur, ran smuggling operations in the Louisiana Bayou during the 18th century.
- Houngan (San Danje): A mysterious individual living in the Louisiana Bayou during the 18th century.
- Carlos Dominguez: A Spanish ship captain, who was active in New Orleans during the late 18th century.
- Alsoomse: A Native American woman of Shawnee ancestry, who lived a very solitary life as a child, subsequently causing her to be considered more independent than those around her.
- Emily Burke: A pioneer during the American Revolutionary War.
- Fillan McCarthy: A thief who, just like his older sister Gillian, learned the ways of the streets in order to survive. Unlike his sister, Fillan chose to steal for a living, while his sister became a seductress.
- John O'Brien: An Irish workman who traveled to America to start a new life, as he found the Templars to be a constant threat in his homeland.
- David Clutterbuck: A gunnery officer on the Assassin vessel the Aquila, serving alongside his brother Richard.
- Richard Clutterbuck: A gunnery officer aboard the Assassin vessel the Aquila, alongside his brother David.
- Amanda Bailey: An innkeeper living in Martha's Vineyard during the American Revolution.
- David Walston: A blacksmith who resided within the Davenport Homestead during the American Revolution.
- Otho Williams: A Colonel in the Continental Army during the American Revolutionary War.
- Benjamin Franklin: One of the Founding Fathers of the United States, and a noted polymath. He was a leading author, printer, political theorist, politician, postmaster, scientist, musician, inventor, satirist, civic activist, statesman, and diplomat.
- Samuel Adams: An American statesman, political philosopher, and a member of the Patriot Sons of Liberty group during the American Revolution.
- Paul Revere: An American silversmith and a leading figure in the Sons of Liberty. He was a member of the Continental Congress and a participant in the Boston Tea Party.
- Israel Putnam: An American general in the Continental Army, who fought with distinction at the Battle of Bunker Hill during the American Revolutionary War.
- Benjamin Tallmadge: A member of the United States House of Representatives during the American Revolutionary War. He also organized the spy circle, the Culper Ring, under the orders of General George Washington in the summer of 1778, during the British occupation of New York at the height of the American Revolutionary War.
- Gilbert du Motier, Marquis de Lafayette: A French aristocrat and military officer born in south central France. During the American Revolutionary War, Lafayette served as a Major-General in the Continental Army under George Washington.
- George Washington: The Commander-in-Chief of the Continental Army in the American Revolutionary War from 1775 to 1783, and served as the first President of the United States from 30 April 1789 to 4 March 1797.
- François de la Serre: The Grand Master of the Parisian Rite of the Templar Order during the late 18th century. He was the father of Élise de la Serre and adoptive father of Arno Dorian.
- Élise de la Serre: A French noblewoman and Templar, as well as the adoptive sister and lover of the Assassin Arno Dorian.
- Donatien Alphonse François, Marquis de Sade: A French aristocrat, revolutionary politician, philosopher and author famous for his libertine sexuality.
- Théroigne de Méricourt: A Belgian singer, orator and organizer active in Paris at the time of the French Revolution.
- Chevalier d'Éon: A famous French aristocrat, transvestite, diplomat, spy, dueler and a soldier in the French Army during the Seven Years' War.
- Georges Danton: A French lawyer and a leading figure of the early French Revolution.
- Marie-Anne Paulze Lavoisier: A French chemist and the wife of Antoine Lavoisier, acting as his lab assistant and contributing to his work.
- Antoine Lavoisier: A French nobleman and chemist, who made significant contributions to the science of combustion, including the identification of oxygen and hydrogen, and his pioneering work on the list of elements.
- Napoleon Bonaparte: A Corsican military and political leader who ruled first as the First Consul of France from 1799 to 1804, then as Emperor of the French from 1804 to 1815.
- Thomas-Alexandre Dumas: A French general during the French Revolutionary Wars.
- Jean-François Champollion: A French scholar, philologist and orientalist as well as the decipherer of the Egyptian hieroglyphs.
- Larose: A Canadian citizen, and an associate of Ludger Duvernay.
- Edmund Bailey O'Callaghan: An Irish doctor and journalist living in Montréal, Canada.
- Raza Soora: A mute Indian servant that served Hamid, the Mentor of the Indian Brotherhood of Assassins, before being purchased by Arbaaz Mir.
- Clara O'Dea: A young British girl that oversaw a network of street urchins and pickpockets in London during the mid-19th century.
- Nigel Bumble: A member of the Assassin-affiliated gang called the Rooks, which were active during the mid-19th century.
- Agnes MacBean: The Scottish owner of a private train she affectionately referred to as "Bertha".
- Ned Wynert: A transgender businessman from the United States who was active in London during the 19th century.
- Frederick Abberline: The Chief Inspector for the London Metropolitan Police Service from 1890 to 1892.
- Alexander Graham Bell: A Scottish-born scientist, inventor, engineer and innovator who was credited with inventing the first practical telephone.
- Charles Darwin: An English naturalist and geologist, best known for his contributions to evolutionary theory.
- Charles Dickens: An English novelist and social critic, regarded as the greatest novelist of the Victorian era.
- Florence Nightingale: An English social reformer and statistician, as well as the founder of modern nursing.
- Karl Marx: A German philosopher, economist, journalist and sociologist considered the founder of the ideology of Marxism.
- Frank Morris: A British worker and an acquaintance of the philosopher Karl Marx in the 19th century.
- Benjamin Disraeli: A British politician and writer who served as Prime Minister of the United Kingdom twice.
- Duleep Singh: The last Maharaja of the Sikh Empire and the youngest son of Ranjit Singh, ruling from 1843 to 1846.
- Victoria, Queen of the United Kingdom: The Queen of the United Kingdom of Great Britain and Ireland from 1837, and Empress of India from 1876, until her death.
- Nellie: One of the prostitutes that worked for the Assassin Jacob Frye.
- Vladimir Lenin: A Russian Marxist revolutionary and communist politician who led the October Revolution of 1917. He was the founder and leader of the Soviet Union from 1917 until his death in 1924.
- Nikola Tesla: A Serbian American inventor, and a mechanical and electrical engineer.
- Anastasia Nikolaevna of Russia: A Russian princess, the fourth and youngest daughter of Tsar Nicholas II and last survivor of the Romanov family.
- Leon Trotsky: A Marxist revolutionary and theorist, a Soviet politician, and the founder of the Red Army. He was Templar ally.
- John Edgar Hoover: The first Director of the Federal Bureau of Investigation of the United States.
- Sergey Fedorovich Oldenburg: A Russian ethnographer and activist in the Kadet Party.
- Fedor Tokarev: A Russian weapons designer and deputy of the Supreme Soviet of the Soviet Union from 1937 to 1950.
- Sir Winston Churchill: A British statesman who was the Prime Minister of the United Kingdom from 1940 to 1945 and again from 1951 to 1955.
- Barthel Schink: A member of the Edelweiss Pirates, active in Cologne.
- John Standish: A Sage and member of the Instruments of the First Will.
- Robert Fraser: An employee at Abstergo Entertainment, tasked with reliving the genetic memories of Arno Dorian, a French Assassin living during the French Revolution.
- Abstergo Entertainment research analyst: A former employee of Abstergo Entertainment that was assigned to the Sample 17 Project.
- Jot Soora: A computer programmer at MysoreTech, and a descendant of Raza Soora, the mute servant of the Indian Assassin Arbaaz Mir.