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Aunt May is a  supporting character from Spider-Man: Into the Spider-Verse. She was voiced by Lily Tomlin who also voiced Ms. Valerie Frizzle.

Personality

She is a native from Miles Morales 'universe. She is the aunt of the Peter Parker of that universe, who dies in battle against the Kingpin.

Due to most Spider-Men in the Multiverse being related to or knowing an Aunt May personally, the Spider-Men that arrive at Miles Universe (Spider-Man Noir, Spider-Ham, Peni Parker, and Gwen Stacy) take refuge in Aunt May's house. Later on, so do Miles as well as the Peter Parker from the main universe. May is visibly disappointed at how much Peter B. let himself go, both physically and mentally.

May, as it turns, has a brilliant scientific mind that she had been using to aid her nephew in battle, and she uses it to aid the other Spider-Men. She provides Miles with his costume and with a set of webshooters that she built herself.

May is a former friend of Doctor Octopus, as evidenced by the fact that May calls Doctor Octopus "Liv" (only Octopus' friends call her "Liv"). It's likely that Olivia Octavius' turn to evil is what ended their friendship.

May is kind, friendly, gentle and wise.

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          Spider-Man Logo.png Heroes

Sam Raimi series
Spider-Man: Peter Parker/Spider-Man | Mary Jane Watson | Harry Osborn/New Goblin | Aunt May | General Slocum | Captain Stacy | Curt Connors | Robbie Robertson | Betty Brant | Uncle Ben | Flash Thompson
Spider-Man 2: John Jameson
Spider-Man 3: Gwen Stacy

Marc Webb series
The Amazing Spider-Man: Peter Parker/Spider-Man | Gwen Stacy | George Stacy | Harry Osborn | Aunt May | Curt Connors/Lizard | Uncle Ben
The Amazing Spider-Man 2: Felicia Hardy

Marvel Cinematic Universe
Spider-Man: Homecoming: Peter Parker/Spider-Man | May Parker | Michelle Jones | Happy Hogan | Tony Stark/Iron Man | Ned Leeds | Betty Brant | Sue Lorman | Liz Toomes | Aaron Davis | Flash Tomson | Brad Davis | F.R.I.D.A.Y.
Spider-Man: Far From Home: Nick Fury | Maria Hill | Skrulls (Talos & Soren)

Spider-Verse
Into the Spider-Verse: Miles Morales/Spider-Man | Peter Parker | Spider-Gwen | Spider-Man Noir | Peni Parker | Spider-Ham | Aunt May | Ultimate Spider-Man

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Animated Features
Stuart Little | Snowbell | Reeko | George Little | DJ Walters | Chowder | Jenny Bennett | Horace Nebbercracker | Reginald Skullinski | Zee | Boog | Elliot | Mr. Weenie | Giselle | Buddy | McSquizzy | Serge and Deni | Ian | Ranger Beth | Cody Maverick | Lani Aliikai | Chicken Joe | Big Z | Tank Evans | Fifi | Flint Lockwood | Sam Sparks | Steve the Monkey | Tim Lockwood | Brent McHale | Officer Earl Devereaux | Manny | Ursa | Alistair | Doug | Gisela and Giselita | Dracula | Mavis Dracula | Jonathan Loughran | Frankenstein | Murray | Wayne | Griffin | Eunice | Wanda | Werewolf Kids | Winnie | Wilbur | Blobby | Martha | The Pirate Captain | Barry | Dennis | Vlad Dracula | Wally | Wilson | Whoopty | Wade | Weepy | Wanye | Red | Chuck | Bomb | Mighty Eagle | Matilda | Terence | Stella | Dahlia | Willow | Poppy | Gale | Hal | Bubbles | The Blues | Hatchlings | King Leonard Mudbeard | Judge Peckinpah | Cyrus | J.C. | Mr. McMahon | Paige | Hunter | The Undertaker | Gene | Jailbreak | Hi-5 | Alex | Bo | Dave | Ruth | Mary | Joseph | Abby | Deborah | Cyrus | Felix | Edith | Leah | Zach | Ericka Van Helsing | Tinkles | Miles Morales | Peter Parker | Gwen Stacy | Spider-Man Noir | Peni Parker | Spider-Ham | Aunt May | Ultimate Spider-Man | Silver | Courtney | Garry Pig | Zeta | Debbie | Glenn | Ella | Roxanne | Katie Mitchell | Rick Mitchell | Linda Mitchell | Aaron Mitchell | Monchi | Dr. Mark Bowman | Eric | Deborahbot 5000 | Abbey Posey | Hailey Posey | Jim Posey

Live-Action Films
Peter Venkman | Ray Stantz | Egon Spengler | Winston Zeddemore | Dana Barrett | Louis Tully | Janine Melnitz | Slimer | Alan Parrish | Sarah Whittle | Judy Shepherd | Peter Shepherd | Carl Bentley | Matilda Wormwood | Jennifer Honey | Steven Kovacs | Robin Harris | Agent J | Agent K | Agent L | Zed | Worms (MIB) | Frank the Pug | Jack Jeebs | Stuart Little | Snowbell | George Little | Spider-Man | Mary Jane Watson | Harry Osborn | Uncle Ben | Aunt May | General Slocum | Robbie Robertson | Betty Brant | Flash Thompson | Margalo | Laura Vasquez | John Jameson | Max | Sharkboy | Lavagirl | Danny | Walter | Lisa | Gwen Stacy | Griffin | Chappie | Deon Wilson | Ninja | Amerika | Yolandi | R.L. Stine | Zach Cooper | Hannah Stine | Champ | Alex Vreeke | Spencer Gilpin | Anthony Johnson | Bethany Walker | Martha Kaply | Sarah Quinn | Sonny Quinn | Sam Carter | Peter Rabbit | Benjamin Bunny | Flopsy Rabbit | Cottontail Rabbit | Mopsy Rabbit | Thomas McGregor | Bea | Tommy Brock | Jemima Puddle-Duck

See Also
Ghostbusters Heroes | Hotel Transylvania Heroes | MIB Heroes | Sony Pictures Animation Heroes | Spider-Man Heroes | The Mitchells vs. the Machines Heroes

           Sony Pictures Animation logo.png Heroes

Animated Features
Boog | Elliot | Mr. Weenie | Giselle | Buddy | McSquizzy | Serge and Deni | Ian | Ranger Beth | Cody Maverick | Lani Aliikai | Chicken Joe | Big Z | Tank Evans | Fifi | Flint Lockwood | Sam Sparks | Steve the Monkey | Tim Lockwood | Brent McHale | Officer Earl Devereaux | Manny | Ursa | Alistair | Doug | Gisela and Giselita | Dracula | Mavis Dracula | Jonathan Loughran | Frankenstein | Murray | Wayne | Griffin | Eunice | Wanda | Werewolf Kids | Winnie | Wilbur | Blobby | Martha | The Pirate Captain | Barry | Dennis | Vlad Draucla | Wally | Wilson | Whoopty | Wade | Weepy | Wanye | Red | Chuck | Bomb | Mighty Eagle | Matilda | Terence | Stella | Dahlia | Willow | Poppy | Gale | Hal | Bubbles | The Blues | Hatchlings | King Leonard Mudbeard | Judge Peckinpah | Cyrus | J.C. | Mr. McMahon | Paige | Hunter | The Undertaker | Gene | Jailbreak | Hi-5 | Alex | Bo | Dave | Ruth | Mary | Joseph | Abby | Deborah | Cyrus | Felix | Edith | Leah | Zach | Ericka Van Helsing | Tinkles | Miles Morales | Peter Parker | Gwen Stacy | Spider-Man Noir | Peni Parker | Spider-Ham | Aunt May | Ultimate Spider-Man | Silver | Courtney | Garry Pig | Zeta | Debbie | Glenn | Ella | Roxanne | Katie Mitchell | Rick Mitchell | Linda Mitchell | Aaron Mitchell | Monchi | Dr. Mark Bowman | Eric | Deborahbot 5000 | Abbey Posey | Hailey Posey | Jim Posey | Jade

Live-Action Movies
R.L. Stine | Zach Cooper | Hannah Stine | Champ | Sarah Quinn | Sonny Quinn | Sam Carter | Peter Rabbit | Benjamin Bunny | Flopsy Rabbit | Cottontail Rabbit | Mopsy Rabbit | Thomas McGregor | Bea | Tommy Brock | Jemima Puddle-Duck

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