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Dr. Mario is a doctor-costumed version of Mario that was the star of his eponymous game for the NES, Game Boy, and SNES, in which he must use his Megavitamins to defeat Viruses. He also stars in Dr. Mario 64 for the Nintendo 64, Dr. Mario and Puzzle League for the Game Boy Advance, Dr. Mario Online Rx, which is a game for Wii's WiiWare and Dr. Mario Express, which is a game for the DSi's DSiWare. He was introduced to the Super Smash Bros. series in Super Smash Bros. Melee, where he is an unlockable character. However, he is not included in Super Smash Bros. Brawl, and then included once again as an unlockable character in Super Smash Bros. for Nintendo 3DS and Wii U, where he uses his Melee move set once again, plus a Final Smash: Dr. Finale. It is the same as Mario Finale, but instead of Fireballs, Dr. Mario launches two giant-sized Megavitamins. Dr. Mario once again returns in Super Smash Bros. Ultimate as an unlockable character once again.

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Trivia

  • In his early appearances, Dr. Mario wore a plain white shirt, but in his later appearances since Dr. Mario 64, Dr. Mario wears a red necktie.
  • In Super Mario Odyssey, there is a Dr. Mario costume for Mario to wear.
  • In the Dr. Mario games, Dr. Mario's pants are white, but in Super Smash Bros. Melee, Super Smash Bros. for Nintendo 3DS/Wii U and Super Smash Bros. Ultimate, his pants are black.

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           Nintendo All-Star Heroes

Super Mario Bros. franchise
Mario | Luigi | Princess Peach | Princess Daisy | Bowser | Yoshi | Rosalina | Lumas | Captain Toad | Toadette | Pauline | Cappy | Paper Mario | Goombella | Dr. Mario | Mallow | Geno

Wario series
Wario | Waluigi | Ashley & Red | Mona | Kat & Ana

Donkey Kong series
Donkey Kong | Diddy Kong | Dixie Kong | Kiddy Kong | Tiny Kong | Lanky Kong | Chunky Kong | Funky Kong | Candy Kong | Cranky Kong

The Legend of Zelda series
Link | Princess Zelda | Sheik | Impa | Young Link | Toon Link | Tetra | Midna | Tingle | Lana | Linkle

Metroid series
Samus Aran | Zero Suit Samus | Rundas

Kid Icarus series
Pit | Palutena | Dark Pit | Magnus | Viridi | Phosphora

Star Fox series
Fox McCloud | Falco Lombardi | Slippy Toad | Peppy Hare | Krystal | James McCloud

Fire Emblem series
Marth | Caeda | Jagen | Draug | Wrys | Navarre | Merric | Minerva | Tiki | Alm | Celica | Sigurd | Deidre | Seliph | Leif | Roy | Lilina | Eliwood | Lyndis | Hector | Ninian | Nils | Eirika | Ephraim | Seth | L'Arachel | Ike | Titania | Mist | Soren | Sothe | Elincia | Chrom | Robin | Lucina | Lissa | Tharja | Corrin | Azura | Jakob | Ryoma | Hinoka | Takumi | Sakura | Xander | Camilla | Leo | Elise

Kirby series
Kirby | King Dedede | Meta Knight | Bandana Waddle Dee | Adeleine | Ribbon | Knuckle Joe | Magolor | Susie Haltmann

Pokémon franchise
Pichu | Pikachu | Charizard | Jigglypuff | Meowth | Ditto | Eevee | Mewtwo | Lugia | Blaziken | Gardevoir | Piplup | Lucario | Zekrom | Braixen | Greninja | Goomy | Decidueye | Incineroar | Vulpix (Alolan) | Lycanroc | Cynthia

Retro series
Sheriff | Bubbles | Ice Climbers | Little Mac | Duck Hunt | R.O.B. | Mr. Game and Watch | Sukapon | Excitebiker | Takamaru

Earthbound/MOTHER series
Ninten | Ana | Queen Mary | Ness | Paula Jones | Jeff Andonuts | Poo | Lucas | Claus | Flint | Hinawa | Kumatora | Duster | Boney

F-Zero series
Captain Falcon | Samurai Goroh | Jody Summer

Animal Crossing franchise
Villager | Isabelle

Pikmin series
Olimar | Pikmin | Louie | The President | Koppaites

Chibi-Robo series
Chibi-Robo

Custom Robo
Ray

Xenoblade series
Shulk | Fiora | Dunban | Riki | Reyn | Sharla | Elma | Rex | Pyra | Mythra | Tora | Zeke

Golden Sun series
Isaac | Jenna | Garet | Ivan | Mia | Felix | Matthew

Splatoon series
Inkling | Squid Sisters | Cap'n Cuttlefish | Off the Hook | Agent 3 | Agent 8

ARMS series
Spring Man | Ribbon Girl | Min Min | Ninjara | Twintelle | Master Mummy | Mechanica | Lola Pop | Max Brass

            Super Mario Bros Logo Heroes

Marioverse
Mario | Luigi | Princess Peach | Princess Daisy | Bowser | Rosalina | Lumas | Toad (Blue Toad | Yellow Toad | Toadsworth | Toadette | Captain Toad) | Pauline | F.L.U.D.D. | Cappy | Tiara | Dr. Mario | Dr. Luigi | Professor Elvin Gadd | Madame Clairvoya | MC Ballyhoo | Lakitu | Poochy | Kylie Koopa | Prince Peasley | Prince Dreambert | Mallow | Geno | Alex | Nina | Harry | Kate | Azalea | Joe | Sherry | Kid |

Yoshi's Colors
Green | Pink | Light Blue | Yellow | Orange | Purple | Brown | Red | Blue | Black | White |

DKverse
Kong Family (Cranky Kong | Donkey Kong | Donkey Kong Jr. | Diddy Kong | Dixie Kong | Funky Kong | Tiny Kong | Candy Kong | Lanky Kong | Chunky Kong | Swanky Kong | Wrinkly Kong) | Klubba | K. Lumsy | Xananab | Rambi the Rhinoceros | Squawks the Parrot | Enguarde the Swordfish | Rattly the Rattlesnake | Expresso the Ostrich | Ellie the Elephant | Squitter the Spider |

Warioverse
Wario | Waluigi | Ashley | Mona | Kat & Ana | Jimmy T. | Dribble & Spitz | 5-Volt | 9-Volt | 18-Volt | Dr. Crygor | Orbulon | Mike | Penny Crygor | Young Cricket & Master Mantis |

Baby Versions
Baby Mario | Baby Luigi | Baby Yoshi | Baby Peach | Baby Daisy | Baby Wario | Baby Donkey Kong | Baby Rosalina | Baby Luma |

Paperverse
Paper Mario | Paper Luigi | Goombario | Kooper | Bombette | Parakarry | Lady Bow | Watt | Sushie | Lakilester | Goombella | Koops | Madame Flurrie | Yoshi Kid | Vivian | Admiral Bobbery | Ms. Mowz | Tippi | Luvbi | Count Bleck |

Other
Metal Mario | Metal Wario | Link | Little Mac | Duck Hunt | R.O.B. | Mr. Game and Watch | Captain Falcon | Villager | Isabelle | Inkling

           Super Smash Bros. Heroes

Playable Heroes
Banjo & Kazooie | Bayonetta | Bowser | Captain Falcon | Chrom | Cloud Strife | Corrin | Dark Pit | Diddy Kong | Donkey Kong | Dr. Mario | Duck Hunt | Eight | Erdrick | Falco Lombardi | Fox McCloud | Greninja | Ice Climbers | Ike | Incineroar | Inkling | Isabelle | Jigglypuff | Joker | Ken Masters | King Dedede | Kirby | Link | Little Mac | Lucario | Lucas | Lucina | Luigi | Luminary | Mario | Marth | Mega Man | Meta Knight | Mewtwo | Mii | Mr. Game and Watch | Ness | Olimar (Alph) & Pikmin | Pac-Man | Palutena | Pichu | Pikachu | Pit | Pokémon Trainer (Red | Leaf) (Charizard | Ivysaur | Squirtle) | Princess Daisy | Princess Peach | Princess Zelda | Richter Belmont | R.O.B. | Robin | Rosalina & Luma | Roy | Ryu | Samus Aran | Sheik | Shulk | Simon Belmont | Solid Snake | Solo | Sonic the Hedgehog | Terry Bogard | Toon Link | Villager | Wario | Wii Fit Trainer | Yoshi | Young Link | Zero Suit Samus |

Assist Trophies
Alucard | Akira | Ashley | Chef Kawasaki | Dillon | Elec Man | Gray Fox | Guile | Isaac | Isabelle | Jeff | Jill | Kat & Ana | Knuckle Joe | Knuckles the Echidna | Krystal | Little Mac | Lyn | Magnus | Midna | Phosphora | Ray MK III | Riki | Rodin | Saki Amamiya | Samurai Goroh | Shadow the Hedgehog | Sheriff | Shovel Knight | Spring Man | Squid Sisters | Starfy | Sukapon | Takamaru | Tiki | Tingle | Waluigi | White Bomberman | Yuri Kozukata | Zero |

Pokémon
Arceus | Alolan Raichu | Alolan Vulpix | Blastoise | Celebi | Chansey | Charizard | Chespin | Darkrai | Dedenne | Deoxys | Ditto | Eevee | Entei | Fennekin | Gardevoir | Giratina | Ho-Oh | Inkay | Kyogre | Kyurem | Latias and Latios | Lugia | Marill | Meloetta | Meowth | Metagross | Mew | Munchlax | Oshawott | Palkia | Piplup | Snivy | Snorlax | Tapu Koko | Vulpix | Zoroark |

Others
5-Volt | Bass | Beat | Blue Toad | Boney | Bottles | Cappy | Centurions | Cresselia | Dialga | Doc Louis | Dunban | Fiora | F.L.U.D.D. | Hal "Otacon" Emmerich | Ho-Oh | Jinjos | Krystal | Kumatora | Master Hand | Mega Man Series (Mega Man X | Mega Man Volnutt | Mega Man.EXE | Mega Man Star Force) | Mei Ling | Metal Mario | Mumbo Jumbo | Paula | Pauline | Peppy Hare | Pikmin | Poo | Proto Man | Registeel | Reshiram | Roy Campbell | Rush | Sans | Slippy Toad | The Mighty Jinjonator | Toad | Tom Nook | Tooty | Viridi | Yoshis (Blue Yoshi | Light Blue Yoshi | Yellow Yoshi | Pink Yoshi | Purple Yoshi | Red Yoshi) | Zekrom |

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