Dr. Marsha Holloway is a psychologist and Area 52's leading researcher in latent superhuman abilities.
Her first appearance in the film was when she helped Dr. Grant and General Larraby take Jack Shepard back to Area 52. After Jack accepted the deal to train The New Team Zenith for $500,000 she was introduced, she revealed that she's a big fan of Zoom's, and knows of him only through the comic book adaptations of the team's adventures.
In the auditions for The New Team Zenith, Marsha give a high rate for Dylan, Cindy, Summer and Tucker's skills. At first, things do not go well with Jack being bitter about the past and his sarcastic attitude disappointed Marsha. When she was asking Jack why he didn't act like the hero he used to be Jack said that she should stop reading comics which broke Marsha's faith in Jack.
When Jack, Dylan, Cindy, Summer, Tucker and Mr. Pibb took the spaceship and revealed it to the whole world, she got very mad and she had an argument with Jack.
After Jack took the training seriously her faith in him was restored. When The New Team Zenith decided to have a party she helped Summer to get prepeared for it. During the party she was dancing with Jack until General Larraby and his soldiers crashed the party.
When the kids were taken to be blasted with Gamma 13 she neutralize the soldiers with her Rainbow-Colored Wind Blast revealing to the kids that she has superpowers as well. When she, Mr. Pibb and the kids released Jack she reveals her superpower to him and she officially becomes member of The New Team Zenith. During the battle with Concussion she helps Jack to free Connor from the effect of Gamma 13.