Duke encounters Woody and Bo Peep when he was asked for help to rescue Forky. He gets Woody, Buzz, Bo, Giggle McDimples, Ducky and Bunny to the antique store to rescue Forky, but when they run into Gabby Gabby and The Benson, they exit the store and Duke crashes. Woody begs him and everyone to return to the store, but he and everyone leave.
After Woody gives his voice box to Gabby Gabby, Duke returns with the gang and helps Woody return to Bonnie. Woody returns to his gang but he chooses to stay with Bo instead of returning to his Bonnie. After Woody said goodbye his friends, Buzz Lightyear and Forky, Duke and everyone begin their quest in finding lost toys find homes and owners.
- Duke is a parody of the 1975 Evel Knievel Rally Stunt Cycle by the Ideal Toy Company.
- In a mid-credits scene, after hearing that Ducky and Bunny really have laser eyes, Duke exclaims "Woah", which refers to Neo, who was also played by Keanu Reeves.
- After the credits, Duke takes Luxo, Jr.’s place during the Pixar logo, and the winter Combat Carl appears and gets a high five from Duke.