In the episode "Bart-Mangled Banner" they can be seen locked inside the jail of the Ronald Reagan Reeducation Center. They appeared as themselves in the episode "Papa Don't Leech".
After Royce Boss Hogg Lumpkin left Lurleen, he became the manager of the Dixie Chicks and gave them the song "Daddy's Back", and changed the word "Daddy" to "America". He later wanted them to sell out the song and do an advert to the lines "Chevy's Back". Lurleen confronts him and after finding out that Royce stole the song from Lurleen, the Dixie Chicks turn on him, beating him with their instruments.
- Natalie Maines
- Martie Maguire
- Emily Robinson