Tooty is Banjo's younger sister. She has a physical appearance in the first Banjo-Kazooie game and has cameo appearances as pictures in Banjo-Tooie.
Tooty is a brown anthomorphic honey bear with blond hair tied into two pigtails with purple hair bands, claws on her fingers and toes, and large blue eyes. Her clothing consists of a red sleeveless shirt with a big yellow star facing the front and purple stirrup pants.
In Banjo-Kazooie, Tooty lived in a house on Spiral Mountain with her lazy brother Banjo. Unfortunately, she became the target of the evil witch Gruntilda, who kidnaps her because she was jealous of Tooty's adorable cuteness. Banjo and Kazooie set out on an adventure to rescue Tooty before Grunty could succeed in stealing away her beauty for good. If the player gets a game over, It is shown that Gruntilda would have succeeded in stealing her beauty, turning her into a large, ugly, fat green bear with her blonde hair; one of her eyes being larger than the other; her shirt would have shrunk and her pants would have stretched. She was later rescued once Banjo and Kazooie won the Grunty's Furnace Fun mini-game.
Tooty makes a cameo appearance in the world Cloud Cluckooland. Her picture can be found within Guffo's Trashcan on the side of a milk carton. Her photo in Banjo's house survives the destruction and can be seen hanging unevenly on the wall.
Banjo-Kazooie: Nuts & Bolts
Tooty is referenced a few times in this game. In Showdown Town, signs can be found advertising the "Tooty Fruity Fruit Shop" and a scoreboard found in the world Jiggoseum also displays Tooty's name. The Lord of Games makes reference to her by saying one of his rejected world names was "Tootyland" and he mentions Tooty during the endgame quiz.
Super Smash Bros. Ultimate
Tooty makes an appearance as an available Support Spirit, granting the Poison Damage Resist effect, after purchasing a DLC Fighters Pass, and can be acquired in a Spirit Battle where the main fighter (Diddy Kong pink palette) must be defeated to win, the enemy shields often and magic attacks don't have much of an effect; Banjo & Kazooie appear as minions in this battle. Defeating Diddy will earn the players the Tooty Spirit. In the Spiral Mountain stage she is seen running around to different spots where she stops, waves and sticks around before bounding over to another spot.